口服聚酯聚醚疫苗蛋白微球的制备研究

采用本体聚合法合成不同聚醚含量的聚酯聚醚嵌段共聚物聚 ＤＬ 乳酸 聚乙二醇（Ｐｏｌｙ ＤＬ ｌａｃｔｉｄｅ ｂ ｐｏｌｙｅｔｈｙｌｅｎｅｇｌｙｃｏｌ，ＰＥＬＡ）．ＰＥＬＡ及ＰＬＡ包裹人血清白蛋白（ＨＳＡ）微球采用溶剂挥发法双乳液体系（Ｗ１／Ｏ／Ｗ２）制备．微球球形规整，粒径集中在０５～５０μｍ．用ＣＢＢ法检测微球中蛋白含量，蛋白包裹量达２５％，包裹效率近８０％．从双乳液体系中界面张力角度考察了聚合物囊材的性质、稳定剂的种类及Ｗ１／Ｏ的稳定性等对微球粒径及蛋白包裹量的影响．微球体外释放结果表明ＰＥＬＡ蛋白微球的突释现象不明显，释放速度较为恒定．

PREPARATION OF POLY-DL-LACTIDE-b-POLYETHYLENE GLYCOL MICROSPHERES ENCAPSULATING HUMAN SERUM ALBUMIN

The purpose of this article is to explore the methods preparing biodegradable microspheres delivery system of vaccines by oral targeting administration.A series of polymers of DL lactide and polyethylene glycol(PEG) with different contents of PEG were synthesized and the microspheres containing human serum albumin(HSA) were prepared by a solvent evaporation method using a W/O/W emulsion.The scanning electron micrograph of microspheres showed a smooth and spheric surface.The size of microspheres could be controlled between 0 5～10μm,which is essential for microspheres to reach the target sites.The amount of protein in microspheres was 2 5% determined by CBB method and the entrapemnt ratio achieved approximately 80%.It was concluded that the properties of polymers,stabilizer system and solvent in oil phase had great effect on the formation of microspheres,particle size and the protein entrapment ratio.In vitro studies indicated that protein carrying microspheres of PLA had a larger burst effect than that of PELA 10.