壳聚糖亲和磁性毫微粒的制备及其对蛋白质的吸附性能研究

以壳聚糖为包裹材料包埋自制的磁流体 ,制备了具有核 壳结构的磁性毫微粒 ,并偶联色素配基CibacronBlue 3GA(偶联量 1 4 .5μmol/mL)得到了一种新型亲和磁性毫微粒 .结果表明 ,所得亲和磁性微球具有较窄的粒径分布、形状规整 .以牛血清白蛋白 (BSA)和溶菌酶 (Lys)为目标蛋白 ,考察了该亲和磁性毫微粒的吸附性能 ,发现其对BSA和Lys的吸附量分别为 4和 2 8mg/g,吸附行为满足Langmuir吸附等温式 ,且对时间依赖性小而对溶液离子强度敏感 .

THE PREPARATION OF CHITOSAN AFFINITY MAGNETIC NANOPARTICLES AND THEIR ADSORPTION PROPERTIES FOR PROTEINS

Magnetic nanoparticles,containing a magnetic core and a polymer shell, were synthesized by coating magnetic fluid with chitosan. Novel affinity magnetic microspheres were obtained after immobilizing dye\|ligand (Cibacron Blue 3GA)onto the nanoparticles by covalent coupling. Under the preparation conditions, the microspheres had a spherical appearance, narrow distribution of diameter, and 14.5μmol/mL immobilization capacity for reactive dye. The microspheres can be separated by magnetic field. Bovine serum albumin (BSA) and lysozyme (Lys) were used as target proteins to examine the adsorption properties of the adsorbent. Under appropriate conditions, the adsorbent had a capacity of 4 and 28mg/g for BSA and Lys, respectively. Adsorption isotherm could be expressed by the Langmuir adsorption model. Unlike adsorption time, the liquid phase ionic strength affects the adsorption properties dramatically.