New method of identifying morphine in urine samples using nanoparticle-dendrimer-enzyme hybrid system |
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Institution: | 1. Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran Iran;2. Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, PO Box 1651153311, Tehran, Iran;3. National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran |
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Abstract: | The incorporation of morphine (MOR) into the nanoparticle structure is a viable alternative to traditional enzyme usage. It has good biological potential to separate MOR from real urine samples. In this study, a new method of MOR identification in real urine samples was synthesized using the β-glucuronidase-dendrimer poly amidoamine (PAMAM) enzyme hybrid system. Replacing MOR in dendrimer cavities significantly reduces enzyme consumption. The replacement technique is done in dendrimer cavities in two stages as an alternative to β-glucuronidase enzyme and even MOR. In this paper, firstly, PAMAM dendrimer G2 was synthesized based on silica. The β-glucuronidase enzyme was replaced inside its dendrimer cavities and the compound was released into a real urine sample containing MOR. The enzyme was extracted from dendrimer cavities. The MOR- β-glucuronidase enzyme bond broke. In the next stage of the process, free MOR entered the PAMAM dendrimer G2 cavities. MOR was detected in real urine samples. |
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Keywords: | Enzyme Dendrimer Morphine Urine samples Trap |
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