合欢皮中两个新八糖苷的分离鉴定和活性研究

从合欢皮95%乙醇提取物中分离得到2个新的八糖苷(1, 2), 经化学方法与光谱分析将其结构鉴定为3-O-β-D-吡喃木糖基-(1→2)-α-L-吡喃阿拉伯糖基-(1→6)-β-D-吡喃葡萄糖基]-21-O-(6S)-2-反式-2-羟甲基-6-甲基-6-O-β-D-吡喃鸡纳糖基-2,7-辛二烯酸基]-金合欢酸-28-O-β-D-吡喃葡萄糖基-(1→3)-α-L-呋喃阿拉伯糖基-(1→4)]-α-L-吡喃鼠李糖基- (1→2)-β-D-吡喃葡萄糖基酯(1)和3-O-β-D-吡喃木糖基-(1→2)-α-L-吡喃阿拉伯糖基-(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖基]-21-O-(6S)-2-反式-2-羟甲基-6-甲基-6-O-β-D-吡喃鸡纳糖基-2,7-辛二烯酸基]-金合欢酸-28-O-β-D-吡喃葡萄糖基-(1→3)-α-L-呋喃阿拉伯糖基-(1→4)]-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖基酯(2), 分别命名为合欢皂苷J₂₅ (1, Julibroside J₂₅)和合欢皂苷J₂₂ (2, Julibroside J₂₂). 1和2在体外对4种人癌细胞增殖有明显的抑制作用.

Structures and Activity of Two Novel Saponins from Albiza julibrissin

Two triterpene saponins acylated with monoterpenoid were isolated from the stem barks of Albizia julibrissin. Their structures were identified as 3-O--D-xylopyranosyl-(1→2)--L-arabinopyranosyl- (1→6)-6--D-glucopyrano-syl-21-O-(6S)-2-trans-2-hydroxymethyl-6-methyl-6-O-D-quinovopyranosyl- 2,7-octadienoyl]-acacic acid-28-O--D-glucopyranosyl-(1→3)-α-2-arabinofuranosyl-(1→4)-α-L-rhomno- pyranosyl-(1→2)--D-glucopyranosylester (1) and 3-O--D-xylopyranosyl-(1→2)--L- arabinopyranosyl- (1→6)--D-2-deoxy-2-acetamidoglucopyranosyl-21-O-(6S)-2-trans-2-hydroxymethyl-6-methyl-6-O--D- quinovopyranosyl-2,7-octadienoyl]-acacic acid-28-O--D-glucopyranosyl-(1→3)--L-arabinofuranosyl- (1→4)--L-rhamnopyranosyl-(1→2)--D-glucopyranosyl ester (2) based on chemical and spectroscopic methods, respectively, named as Julibrosides J₂₅ and J_22. Julibrosides J₂₅ and J₂₂ showed marked activities against four kinds of cancer cell lines in a concentration of 100 g/mL assayed by SRB method.