Enhanced surface plasmon resonance by Au nanoparticles immobilized on a dielectric SiO2 layer on a gold surface |
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Authors: | Jaeyeon Jung Jonghwan Lee Jinho Hyun |
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Institution: | a Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea b National Instrumentation Center for Environmental Management, Seoul National University, Seoul 151-742, Republic of Korea c Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea |
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Abstract: | This paper introduces strategies for enhancement of a surface plasmon resonance (SPR) signal by adopting colloidal gold nanoparticles (AuNPs) and a SiO2 layer on a gold surface. AuNPs on SiO2 on a gold surface were compared with an unmodified gold surface and a SiO2 layer on a gold surface with no AuNPs attached. The modified surfaces showed significant changes in SPR signal when biomolecules were attached to the surface as compared with an unmodified gold surface. The detection limit of AuNPs immobilized on a SPR chip was 0.1 ng mL−1 for the prostate-specific antigen (PSA), a cancer marker, as measured with a spectrophotometer. Considering that the conventional ELISA method can detect ∼10 ng mL−1 of PSA, the strategy described here is much more sensitive (∼100 fold). The enhanced shift of the absorption curve resulted from the coupling of the surface and particle plasmons by the SiO2 layer and the AuNPs on the gold surface. |
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Keywords: | Enhancement Gold nanoparticles (AuNPs) Prostate-specific antigen (PSA) Silica (SiO2) Surface plasmon resonance (SPR) |
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