Chromatography of leaf chloroplast pigments on ion-exchange papers |
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Authors: | Joseph Sherma Harold H Strain |
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Institution: | Department of Chemistry, Lafayette College, Easton, Pa. 18042 U.S.A.;Chemistry Division, Argonne National Laboratory, Argonne, Ill. 60439 U.S.A. |
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Abstract: | For the separation of leaf carotenoid pigments, some ion-exchange papers are more effective than pure cellulose papers. Separations of the major pigments on ion-exchange paper are superior to those obtained by uni-dimensional paper chromatography. For green-leaf extracts, ion-exchange papers offer no advantage. Ion-exchange columns do not separate the chloroplast pigments. On ion-exchange paper, there is no major yellow pigment in addition to neoxanthin, violaxanthin, lutein, and carotene. |
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