Fast analysis of soy isoflavones by high-performance liquid chromatography with monolithic columns |
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Authors: | Rostagno Mauricio A Palma Miguel Barroso Carmelo G |
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Institution: | Departamento de Química Analitica, Facultad de Ciencias, Universidad de Cádiz, P.O. Box 40, 11510 Puerto Real, Cádiz, Spain |
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Abstract: | A fast method using high-performance liquid chromatography based on two monolithic columns has been developed for the simultaneous determination of isoflavones extracted from soybeans and derived foods. The 12 main isoflavones were resolved in 10 min in two coupled monolithic columns working at 35 °C using a elution gradient of acidified water (0.1% acetic acid) and methanol (0.1% acetic acid) at a flow rate of 5 mL min−1. Retention time and relative area standard deviations were below 1% for all isoflavones. The method developed was successfully applied to several soy food samples and spiked samples. Total isoflavone concentration in sampled soy foods ranged from 34.28 mg L−1 to 4.29 mg g−1. |
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Keywords: | High-performance liquid chromatography Monolithic columns Isoflavones Soybeans Soy foods |
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