In-gel NHS-propionate derivatization for histone post-translational modifications analysis in Arabidopsis thaliana |
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Authors: | Jiajia Chen Jun Gao Maolin Peng Yi Wang Yanyan Yu Pengyuan Yang Hong Jin |
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Institution: | 1. Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China;2. State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China;3. China Novartis Institutes for BioMedical Research Co. Ltd., Building 8, Lane 898 Halei Road, Shanghai 201203, China |
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Abstract: | Post-translational modifications (PTMs) on histone are highly correlated with genetic and epigenetic regulation of gene expression from chromatin. Mass spectrometry (MS) has developed to be an optimal tool for the identification and quantification of histone PTMs. Derivatization of histones with chemicals such as propionic anhydride, N-hydroxysuccinimide ester (NHS-propionate) has been widely used in histone PTMs analysis in bottom-up MS strategy, which requires high purity for histone samples. However, biological samples are not always prepared with high purity, containing detergents or other interferences in most cases. As an alternative approach, an adaptation of in gel derivatization method, termed In-gel NHS, is utilized for a broader application in histone PTMs analysis and it is shown to be a more time-saving preparation method. |
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Keywords: | Epigenetic Histone post-translational modifications In-gel derivatization Multiple reaction monitoring quantification |
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