An effective colorimetric and ratiometric fluorescent probe for bisulfite in aqueous solution |
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Authors: | Xi Dai Tao Zhang Zhi-Fang Du Xiang-Jian Cao Ming-Yu Chen Sheng-Wen Hu Jun-Ying Miao Bao-Xiang Zhao |
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Institution: | 1. Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China;2. Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China;3. Taishan College, Shandong University, Jinan 250100, PR China |
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Abstract: | We have developed the first two-photon colorimetric and ratiometric fluorescent probe, BICO, for the detection of bisulfite (HSO3−) in aqueous solution. The probe contains coumarin and benzimidazole moieties and can detect HSO3− based on the Michael addition reaction with a limit of detection 5.3 × 10−8 M in phosphate-buffered saline solution. The probe was used to detect bisulfite in tap water, sugar and dry white wine. Moreover, test strips were made and used easily. We successfully applied the probe to image living cells, using one-photon fluorescence imaging. BICO overcomes the limitations in sensitivity of previously reported probes and the solvation effect of bisulfite, which demonstrates its excellent value in practical application. |
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Keywords: | Ratiometric fluorescent Two-photon Bisulfite Cell imaging |
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