| Oxazine750对平行四股螺旋结构的识别与稳定作用 |
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| 引用本文： | 陈名利,任劲松.Oxazine750对平行四股螺旋结构的识别与稳定作用[J].应用化学,2010,27(9):1061-1064. |
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| 作者姓名： | 陈名利 任劲松 |
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| 作者单位： | (中国科学院长春应用化学研究所 稀土资源与利用国家重点实验室 长春 130022) |
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| 摘 要： | 以dTG4T]4、dG4T4G4]2和c-myc序列为代表，采用竞争平衡透析、紫外光谱和圆二色光谱研究了oxazine750对四股螺旋结构的识别与稳定作用。 结果表明，oxazine750能够更好的结合在平行四股螺旋上，而对反平行四股螺旋结合较少。 热变性实验表明，oxazine750使dTG4T]4形成的平行结构的四股螺旋的解旋温度提高了11 ℃，对c-myc形成的平行四股螺旋的解旋温度甚至提高了40 ℃。 而对dG4T4G4]2形成的反平行结构解旋温度降低了近8 ℃。
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| 关 键 词： | 四股螺旋 小分子 竞争平衡透析 |
| 收稿时间： | 2009-12-18 |
| 修稿时间： | 2010-01-28 |
Parallel G-quadruplex Recognition and Stabilization by Oxazine750 |
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| CHEN Ming-Li,REN Jin-Song.Parallel G-quadruplex Recognition and Stabilization by Oxazine750[J].Chinese Journal of Applied Chemistry,2010,27(9):1061-1064. |
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| Authors: | CHEN Ming-Li REN Jin-Song |
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| Institution: | (Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022) |
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| Abstract: | The bindings of small molecular oxazine750 to dTG4T]4, dG4T4G4]2 and c-myc sequences was characterized by means of circular dichroism, thermal denaturation, and so on. Oxazine750 was found to bind preferentially and strongly to parallel G-quadruplex. It can effectively stabilize the parallel G-quadruplex and destabilize the antiparallel G-quadruplex. The melting temperature of dTG4T]4 or c-myc is increased by about 11 ℃ or 40 ℃ by oxazine750, respectively. Under the same condition, oxazine750 decreased the melting temperature of dG4T4G4]2 by about 8 ℃. |
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| Keywords: | G-quadruplex small molecular competition dialysis |
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