纤维素经离子液体预处理酶解糖化研究

利用离子液体氯化1－丁基－3－甲基－咪唑（C4mim]Cl）进行纤维素预处理。实验表明，纤维素经离子液体预处理后聚合度下降；糖化速度随预处理温度增加呈现先增大后下降的趋势，在90℃出现最大值；延长预处理时间和采用乙醇作为沉淀剂，可促进酶解糖化。与未处理的纤维素相比，经离子液体预处理后纤维素的糖化速度可提高70％。根据扫描电子显微镜（SEM）观察，纤维素出现了明显的解聚。

Cellulose saccharification using an ionic liquid pretreatment

Cellulose was pretreated using the IL, 1-n-butyl-methylimidazolium chloride(C4mim]Cl). The experimental results showed that DP(degree of polymerization) of cellulose is diminished by C4mim]Cl pretreatment. With the increase of pretreatment temperature, the saccharification rate increases at first and later decreases and it reaches the fastest at 90ºC. Extending pretreatment time or using ethanol as anti-solvent can enhance saccharification kinetics. The saccharification rate increased by 70% as compared to that of untreated cellulose. SEM studies show that the cellulose depolymerized into fragments by C4mim]Cl.