商品化血糖仪用于乙型肝炎病毒的便携式体外分子诊断

为解决当前乙型肝炎病毒(HBV)基因检测技术在一定程度上存在成本高、操作繁琐、误诊率高等问题,在此提出了一种便携式生物传感平台用于HBV基因的超灵敏检测,可检出低至2 copies/μL的HBV基因.首次通过设计针对HBV检测序列的环介导等温扩增(LAMP)反应,实现了在40 min之内对HBV基因型单拷贝基因的检测....

A Commercial Glucose Meter for Portable in vitro Molecular Diagnosis of Hepatitis B Virus

The current detection methods for hepatitis B virus （HBV） require high cost， complicated manipulation and are usually with high misdiagnosis rate. All the above issues have inspired us to develop a portable biosensing platform for the HBV detection. The loop-mediated isothermal amplification （LAMP） reaction designed for HBV sequence contributes to the single copy gene detection within 40 min. The immobilization of capture probe F-Probe （FP） on the magnetic beads （MBs） and the modification of FPc （partially complementary to FP） with heat-resistant invertase （Inv） collectively constitute the Inv-FPc signal probe， and the hybridization of Inv-FPc and FP creates the signal transduction probe， which with the HBV LAMP amplicons would induce the replacement of nucleic acid chains， isolating the Inv-FPc from the signal transduction probe. The Inv-FPc signal probe in the solution after magnetic separation would catalyze the transformation of sucrose into glucose that is read by glucometer. The experimental results show that the qualified signal response is in the concentration range of 10~500 nmol/L of HBV gene segments. Almost concordant signal and background are observed when employing Norovirus （NoV） gene as the target. The biosensor presents a perfect “yes/no” signal response in 10% human serum and has a potential to be applied in point of care diagnosis of HBV.