pH和温度敏感型聚合物荧光探针的合成及应用

以萘酰亚胺(NI)、N-异丙基丙烯酰胺(NIPAM)和多面体齐聚倍半硅氧烷(POSS)为原料,通过可逆加成断裂自由基聚合(RAFT)方法,设计合成了具有p H响应和温度敏感的聚合物荧光探针Poly(POSS-NINIPAM)。通过核磁共振波谱仪(NMR)、荧光发射光谱仪和质谱仪(MS)等技术手段表征了Poly(POSS-NINIPAM)的p H和温度的响应性能。结果表明,在p H值5.8~8.0范围内,探针Poly(POSS-NI-NIPAM)的荧光强度变化明显,与p H值呈现良好的线性相关(λem=520 nm,p Ka=6.51),对p H和温度的响应均呈现出良好的循环检测性能,可用于检测活细胞He La的p H值变化。探针Poly(POSS-NI-NIPAM)在检测生物样本中p H/温度变化方面具有潜在的应用前景。

Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM) was synthesized from naphthalimide(NI), N-isopropyl acrylamide(NIPAM) and polyhedral oligomeric silsesquioxane(POSS) as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT). The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM) were characterized by nuclear magnetic resonance spectroscopy(NMR), fluorescence emission spectrometer and mass spectrometry(MS). The results show that Poly(POSS-NI-NIPAM) shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance. The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM) as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.