某些碱性三苯甲烷染料共振瑞利散射法测苯唑西林

在NaOH 溶液中，苯唑西林（OXA）能与某些碱性三苯甲烷染料如甲基紫（MV）、乙基紫（EV）和孔雀石绿（MG）结合，使体系的共振瑞利散射（RRS）急剧增强并产生新的RRS光谱，最大共振光散射峰分别位于333 nm(MV体系)、342 nm(EV体系)和343 nm（MG体系），苯唑西林的质量浓度在0.080～0.60 mg/L(MV体系)、0.040～0.40 mg/L(EV体系和MG体系)时与散射强度呈良好的线性关系，检出限（3σ）分别为0.064 mg/L(MV体系)、0.024 mg/L(EV体系)和0.013 mg/L(MG体系)，其中以孔雀石绿体系最灵敏，以其为例考察了共存物质的影响。 结果表明，方法具有较高的选择性，用于人血清、人尿及市售药物中苯唑西林的测定，结果满意。

Determination of Oxacillin with Some Basic Triphenylmethane Dyes by Resonance Rayleigh Scattering

In sodium hydroxide solution, oxacillin(OXA) can be bound to some basic triphenylmethane dyes such as methyl violet(MV), ethyl violet(EV) or malachite green(MG) to form a new product, resulting in a great enhancement of resonance Rayleigh scattering(RRS) and the appearance of a new RRS spectrum. The maximum RRS peak locates at 333 nm for MV system, 342 nm for EV system and 343 nm for MG system, respectively. The RRS intensity was directly proportional to the OXA concentration in the range of 0.080～0.60 mg/L for MV system, 0.040～0.40 mg/L for EV and MG systems, respectively. The lowest detection limits(3σ) of OXA were 0.064 mg/L for MV system, 0.024 mg/L for EV system, and 0.013 mg/L for MG system, respectively. Among these three systems, the MG system has the highest sensitivity, and the effects of the coexisting substances were investigated for this system. The results indicate that this assay has a good selectivity. The system is applied to the determination of oxacillin in human urine liquid, serum and commercial pharmaceuticals with satisfactory results.