| 荧光光谱法研究茶碱与牛血清白蛋白的相互作用 |
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| 引用本文： | 张红梅,王彦卿,张根成.荧光光谱法研究茶碱与牛血清白蛋白的相互作用[J].应用化学,2006,23(8):866-0. |
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| 作者姓名： | 张红梅 王彦卿 张根成 |
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| 作者单位： | 江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002 |
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| 摘 要： | 荧光光谱法研究茶碱与牛血清白蛋白的相互作用;茶碱;牛血清白蛋白;荧光猝灭;能量转移
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| 关 键 词： | 荧光光谱法研究茶碱与牛血清白蛋白的相互作用 茶碱 牛血清白蛋白 荧光猝灭 能量转移 |
| 文章编号： | 1000-0518(2006)08-0866-05 |
| 收稿时间： | 09 23 2005 12:00AM |
| 修稿时间： | 02 16 2006 12:00AM |
Study of Interaction between Theophylline and Bovine Serum Albumin by Fluorescence Spectroscopy |
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| ZHANG Hong-Mei,WANG Yan-Qing,ZHANG Gen-Cheng.Study of Interaction between Theophylline and Bovine Serum Albumin by Fluorescence Spectroscopy[J].Chinese Journal of Applied Chemistry,2006,23(8):866-0. |
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| Authors: | ZHANG Hong-Mei WANG Yan-Qing ZHANG Gen-Cheng |
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| Abstract: | The interaction between theophylline and bovine serum albumin(BSA) was investigated via fluorescence spectroscopy.The experimental results show that the fluorescence quenching of BSA by theophylline is due to the formation of theophylline-BSA complex through both static quenching and nonradiative energy transfer. The binding site number n and the apparent binding constant K_(A) were measured;the binding distance r and the energy transfer efficiency E between theophylline and BSA were obtained according to fluorescence resonance energy transfer;the effect of theophylline on the conformation of BSA was analyzed by means of(synchronous) fluorescence spectroscopy. |
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| Keywords: | theophylline bovine serum albumin fluorescence quenching energy transfer |
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