共振瑞利散射法对普萘洛尔特效适配体系的手性分析

本文提出了对于手性药物普萘洛尔手性识别和手性分析的新方法。 该方法引用基于氧化石墨烯的指数富集配体系统进化筛选技术(GO-SELEX),经过10轮优化筛选出对心血管药物普萘洛尔有高度亲和力的特效适配体。 然后通过共振瑞利散射光谱法(RRS)对反应体系进行特效性检测,实验表明S-普萘洛尔和R-普萘洛尔有迥然不同的光谱差异,S-普萘洛尔与特效适配体结合后的RRS显著增强,而R-普萘洛尔与适配体结合后的RRS几乎没有变化。 据此可以对心血管药物手性普萘洛尔进行有效的手性识别。 在考察反应体系和实验条件的基础上,可对S-普萘洛尔进行实验检测,同时对外消旋体中的R-普萘洛尔进行计算分析。 实验对S-普萘洛尔的线性范围为5~275 nmol/L,检测限为0.5 nmol/L。 方法应用于外消旋药片的分析检测,结果令人满意。 实验表明,RRS检测特效适配体结合的手性靶标体系会彰显不同的光谱差异,从而可对手性对映体进行手性识别,尤其是可利用其光谱差异实现同时测定的手性分析,方法可在特殊情形下不经分离而同时测定手性对映体,具有推广应用价值。

Chiral Analysis of Propranolol and Its Aptamer System by Resonance Rayleigh Scattering Spectral Method

This paper presents a new method for chiral identification and analysis of propranolol.The method quoted the systematic evolution of ligands by exponential enrichment technique based on graphene oxide(GO-SELEX).The specific aptamer with high affinity to the cardiovascular drug propranolol was selected after ten rounds of optimization and screening.Then resonance Rayleigh scattering spectroscopy(RRS)was used to detect the specificity of the reaction system.The experiment results showed that S-propranolol and R-propranolol had utterly spectral differences.The RRS spectrum of S-propranolol combined with the specific aptamer was significantly enhanced,while the RRS spectrum of R-propranolol's was almost unchanged.Therefore,the chiral propranolol could be effectively recognized.Based on the investigation of the reaction system and experimental conditions,S-propranolol could be tested experimentally and R-propranolol in the racemate can be calculated.The linear range of S-propranolol was 5~275 nmol/L and the detection limit was 0.5 nmol/L.The method was applied to the determination of racemic tablets with satisfactory results.The RRS spectra of chiral targets system binded with specific aptamer could reveal chirality differences and the chiral recognition of chiral enantiomers can be carried out.