基于框架核酸的细胞表面受体配体相互作用研究进展

细胞表面受体与配体之间的特异性相互作用在细胞生物学过程中起着重要作用。然而，与均相溶液不同，受体分子在细胞膜上的分布是非连续的、动态的，因此细胞表面的受体配体相互作用通常呈现复杂的非线性结合模式。框架核酸作为一类具有确定几何形状的DNA纳米支架，可用于多价配体的偶联，为深入揭示受体配体相互作用机制提供了可靠的工具。利用框架核酸纳米分辨率的可寻址特性，可实现对配体数目、间距及空间构象等参数的精确调控，进而研究细胞表面受体配体的结合特性及影响因素，优化结合条件最终实现高效的分子识别及靶向治疗。本文综述了基于框架核酸的细胞表面受体配体相互作用研究进展，通过探讨细胞表面受体配体相互作用的重要影响因素及生物学应用，对该研究领域的发展前景和未来趋势予以展望。

Advances in Receptor?ligand Interactions on Cell Surface Based on Framework Nucleic Acids

The specific interaction between receptor and ligand on cell surface plays an important role in cell biology. However， the distribution of receptor molecules on the cell membrane is discontinuous and dynamically different from that in the homogeneous solutions. The receptor-ligand interactions on the cell surface usually exhibit complex nonlinear binding patterns. In order to reveal the mechanisms， framework nucleic acids， as a class of DNA nanostructures with definite geometric shapes， could be used for the coupling of multivalent ligands. With the addressability at nanoscale， the number， spacing and spatial conformation of ligands are precisely regulated on framework nucleic acids. The binding characteristics between receptor and ligand on cell surface are studied， and efficient molecular recognition and targeted therapy could be achieved by optimizing these influencing factors. In this paper， the research progress of receptor-ligand interactions on cell surface based on framework nucleic acids is reviewed. By discussing the important influencing factors and biological applications， the future development of receptor-ligand interactions on cell surface based on framework nucleic acids is prospected.