4种动物药水提液中3种嘌呤碱HPLC法测定

以甲醇/水为流动相，反相高效液相色谱-紫外检测器测定了乌蛇、蟾蜍、土虫、地龙水提取液中黄嘌呤、次黄嘌呤、腺嘌呤的含量,对样品提取和高效液相色谱条件进行了优化。 黄嘌呤、次黄嘌呤、腺嘌呤的检出限分别为0.012×10-6、0.041×10-6、0.042×10-6 g/mL，样品分析的日内测定相对标准偏差分别小于4.1%、7.3%、4.5%，日间测定相对标准偏差分别小于5.8%、2.9%、3.1%,平均加标回收率为94%～107%。

Determination of Hypoxanthine, Xanthine and Adenine Extracts in Four Animal Drugs by HPLC

Hypoxanthine, xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector. This process of sample preparation was improved and the conditions of HPLC were also optimized. The detection limits of hypoxanthine, xanthine and adenine were 0.012×10-6, 0.041×10-6, 0.042×10-6 g/mL, respectively. The intra-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 4.1%, 7.3% and 4.5%, respectively. The inter-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 5.8%, 2.9% and 3.1%, respectively. The recovery rates of the four samples with added specimen were in a range of 94% to 107%.