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| 竹红菌甲素与血红蛋白相互作用光谱 | |
| 引用本文： | 冯玉英,吴晓红,周家宏,顾晓天,陆天虹,王雪松,张宝文.竹红菌甲素与血红蛋白相互作用光谱[J].应用化学,2005,22(8):895-0. |
| 作者姓名： | 冯玉英 吴晓红 周家宏 顾晓天 陆天虹 王雪松 张宝文 |
| 作者单位： | 1. 南京师范大学,江苏省生物医药功能材料工程研究中心,南京,210097 2. 南京师范大学,江苏省生物医药功能材料工程研究中心,南京,210097;中国科学院长春应用化学研究所,长春 3. 中国科学院理化技术研究所,北京 |
| 基金项目： | 江苏省教育厅自然科学基金(2004191XGQ2B43,04KJD150110)，南京师范大学优秀高层次人才科研启动基金资助项目 |
| 摘 要： | 竹红菌甲素与血红蛋白相互作用光谱;马心血红蛋白;竹红菌甲素;相互作用;UV-Vis吸收光谱;荧光光谱;荧光猝灭动力学常数 |
| 关 键 词： | 竹红菌甲素与血红蛋白相互作用光谱 马心血红蛋白 竹红菌甲素 相互作用 UV-Vis吸收光谱 荧光光谱 荧光猝灭动力学常数 |
| 文章编号： | 1000-0518(2005)08-0895-04 |
| 收稿时间： | 2009-06-29 |
| 修稿时间： | 2004年10月26 |
Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods |
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| FENG Yu-ying,WU Xiao-Hong,ZHOU Jia-hong,GU Xiao-Tian,LU Tian-hong,WANG Xue-Song,ZHANG Bao-wen.Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods[J].Chinese Journal of Applied Chemistry,2005,22(8):895-0. | |
| Authors: | FENG Yu-ying WU Xiao-Hong ZHOU Jia-hong GU Xiao-Tian LU Tian-hong WANG Xue-Song ZHANG Bao-wen |
| Institution: | FENG Yu-Ying~ |
| Abstract: | The interaction of horse heart hemoglobin with Hypocrellin A(HA) was studied by means of (UV-Vis) absorption spectroscopy and fluorescence spectroscropy under physiological condition. In the UV-Vis absorption spectrum, the intensity of the absorption peak of amino acid residues in the hemoglobin molecule decreases and the peak shifts bathochromically due to the hydrogen bonding between the amino acid (residue) in the hemoglobin molecule and HA. The synchronous fluorescence spectra show that the degree of the interaction of HA with different amino acid residues are different. For example, the fluorescence quenching kinetic (constants) of HA for tryptophane and tyrosine residues were 5.510~(12) and 1.710~(12) L/mol, respectively, which clearly demonstrates that the interaction between HA and tryptophane residue is more intense than that between HA and tyrosine residue. |
| Keywords: | horse heart hemoglobin Hypocrellin A interaction UV-Vis absorption spectroscopy fluorescence spectroscopy fluorescence quenching kinetic constant |
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