CdTe/CdS量子点与丝裂霉素的相互作用及其应用

在水相中合成了硫普罗宁(Tiopronin,TP)修饰的CdTe/CdS量子点(TP-CdTe/CdS QDs).利用紫外-可见吸收光谱、荧光光谱研究了TP-CdTe/CdS QDs与丝裂霉素(mitomycin C,MMC)的相互作用机理.在pH=7.6的tris-HCl缓冲溶液介质中,TP-CdTe/CdS QDs与MMC相互作用,使TP-CdTe/CdS QDs的荧光发生猝灭,并且QDs的荧光强度与MMC的浓度有良好的线性关系(r=0.9991),线性范围4.7×10-9～1.2×10-8g/mL,检出限(3σ)为1.4×10-g/mL.此方法快速简便,用于尿样中丝裂霉素的测定,实验结果令人满意.

A Study on the Interactions Between CdTe/CdS Quantum Dots with Mitomycin C and Its Analytical Application

Tiopronin(TP) capped with CdTe/CdS quantum dots(TP-CdTe/CdS QDs) was synthesized in aqueous solution.The paper utilized ultraviolet-visible(UV-Vis) absorption spectra and fluorescence spectrometry to study the interactions between TP-CdTe/CdS QDs and mitomycin C(MMC).With the optimum reaction conditions which was found to be at pH=7.6 with tris-HCl buffer solution,the quenched fluorescence intensity of TP-CdTe/CdS QDs was linearly proportional with the concentration of MMC,correlation coefficient r=0.9991,and the detection limits were in the range of 4.7×10-9~1.2×10-8 g/mL,and the detection limit(3σ) for MMC was 1.4×10-8 g/mL.The quantitative determination of MMC is quick,simple and convenient.This method has been applied to the determination of MMC in urine samples with satisfactory results.