紫外-可见吸收、荧光光谱研究CdTe/CdS量子点与盐酸药根碱的相互作用及其应用

合成了巯基乙酸(TGA)修饰的壳核型CdTe/CdS量子点(TGA-CdTe/CdSQDs).利用紫外-可见光谱吸收、荧光光谱研究TGA-CdTe/CdS QDs与盐酸药根碱(JH)的相互作用机理.在pH值为7.4的tris-HCl缓冲溶液介质中,QDs与JH相互作用后使QDs的荧光呈线性猝灭,并有良好的线性关系(r=0.999 1),线性范围0.011 ～ 10 mg/L,检出限(3σ为3.3×10-3 mg/L,因此可以作为一种快速、简便、定量测定盐酸药根碱的新方法.

Determination of Jatrorrhizine Hydrochloride by Core-shell CdTe/CdS Quantum Dots with Fluorescence Quenching Method

The mercaptoacetic acid(TGA) stabilized core-shell CdTe/CdS quantum dots(TGA-CdTe/CdS QDs) were synthesized using as stabilizer.A new method for the determination of jatrorrhizine hydrochloride has been developed based on the quenching of the fluorescence of TGA-CdTe/CdS QDs at excitation wavelength of 340 nm.The paper utilized fluorescence spectrometry and ultraviolet-visible(UV-Vis) absorption spectra to study the interaction between TGA-CdTe/CdS QDs and jatrorrhizine hydrochloride(JH).Under optimized conditions,in which at the pH value is 7.4 using tris-HCl buffer solution,the quenched fluorescence intensity of TGA-CdTe/CdS QDs was linearly proportional with a correlation coefficient r=0.9991,and the limits of detection were in the range of 0.011～10 mg/L and the detection limit for JH(3σ) was 3.3×10-3 mg/L.JH could be conveniently and quantitatively detemined using this new method.