| 紫外可见分光光度法测定不同产地龙牙百合中皂苷含量 |
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| 引用本文： | 傅春燕,刘永辉,李明娟,王晓慧,唐虹,尹文清.紫外可见分光光度法测定不同产地龙牙百合中皂苷含量[J].广东微量元素科学,2011,18(5):55-58. |
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| 作者姓名： | 傅春燕 刘永辉 李明娟 王晓慧 唐虹 尹文清 |
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| 作者单位： | 傅春燕,李明娟,王晓慧,唐虹,FU Chunyan,LI Mingjuan,WANG Xiaohui,TANG Hong(邵阳医学高等专科学校,湖南,邵阳,422000);刘永辉,LIU Yonghui(湖南省新邵县红十字会医院,湖南,邵阳,422000);尹文清,YIN Wenqing(广西师范大学化学化工学院药用资源化学与药物分子工程教育部重点实验室,广西,桂林,541004) |
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| 摘 要： | 为建立龙牙百合总皂苷的测定方法,并确定不同产地龙牙百合中皂苷的含量,以便控制龙牙百合药材的质量,采用香草醛-高氯酸显色,在535 nm处,利用紫外可见分光光度法测定了10个批次的龙牙百合中总皂苷的含量.结果表明,10个不同产地龙牙百合中皂苷的含量,分别是:8.39%、8.11%、8.24%、7.62%、7.38%、9....
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| 关 键 词： | 龙牙百合 总皂苷 紫外可见分光光度法 含量测定 |
Determination of the Content of Total Saponins in Lilium brownii F E Brown var viridulum Baker.By UV Spectrophotometry |
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| FU Chunyan,LIU Yonghui,LI Mingjuan,WANG Xiaohui,TANG Hong,YIN Wenqing.Determination of the Content of Total Saponins in Lilium brownii F E Brown var viridulum Baker.By UV Spectrophotometry[J].Trace Elements Science,2011,18(5):55-58. |
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| Authors: | FU Chunyan LIU Yonghui LI Mingjuan WANG Xiaohui TANG Hong YIN Wenqing |
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| Institution: | 1.Department of Pharmacy,Shaoyang Junior Medicine College,Shaoyang 422000,China;2.Red Cross Hospital at Xinshao County of Hunan Province,Shaoyang 422000,China;3.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources,Ministry of Educationof China,College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin 541004,China) |
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| Abstract: | To establish a method for the quantitative determination of total saponins in Lilium brownii F E Brown var viridulum Baker by UV spectrophotometry the determination was carried out with chemical reaction,and the total saponins in ten different samples were determined by UV at wavelengths 535 nm.The results showed that the content of the total saponins in ten different samples were 8.39%,8.11%,8.24%,7.62%,7.38%,9.02%,8.83%,8.87%,9.21%,9.17% respectively,and RSDS of the above ten samples were 1.22%,0.87%,0.93%,1.32%,0.86%,1.37%,0.94%,1.39%,1.55%,1.61%.It concluded the method was simple,accurate and could be used in the quantitative determination of total saponins in Lilium brownii F E Brown var viridulum Baker. |
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| Keywords: | Lilium brownii F E Brown var viridulum Baker total flavonoids UV spectrophotometry determination |
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