| Oxidative biomacromolecular damage from novel phthalocyanine |
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| 作者姓名： | 董润安 邱勇 田淑芳 王升启 宋心琦 |
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| 作者单位： | 1. State Key Laboratory of Biomembrane and Membrane Biotechnology，Institute of Zoology，Chinese Academy of Sciences，Beijing 100080，China; 2. Department of Chemistry and Department o |
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| 摘 要： | Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｐｈｔｈａｌｏｃｙａｎｉｎｅｓａｒｅｗｉｄｅｌｙｕｓｅｄｉｎｐｈｏｔｏｄｙｎａｍｉｃｔｈｅｒａｐｙ(ＰＤＴ)ａｎｄｌｉｇｈｔｉｎｄｕｃｅｄｅｌｅｃｔｒｏｎｉｃｄｅｖｉｃｅｓ.Ｔｈｅｙｃａｎｂｅｐｏｔｅｎｔｉａｌｌｙｕｓｅｄｉｎｔｈｅｔｈｅｒａｐｙｏｆｃａｎｃｅｒａｎｄｉｎｔｈｅｒｅｓｔｒａｉｎｔｏｆｈｕｍａｎｉｍｍｕｎｏｌｏｇｙｖｉｒｕｓ(ＨＩＶ),ｅｔｃ.1,2].Ｐｈｏｔｏｓｅｎｓｉｔｉｚｅｄｏｘｉｄａｔｉｏｎｉｓａｐｒｏｃｅｓｓｉｎｗｈｉｃｈｐｈｏｔｏｓｅｎｓｉｔｉｚｅｒｏ…
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| 收稿时间： | 5 June 1997 |
Oxidative biomacromolecular damage from novel phthalocyanine |
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| Runan Dong,Yong Qiu,Shufang Tian,Shengqi Wang,Xinqi Song.Oxidative biomacromolecular damage from novel phthalocyanine[J].Science in China(Chemistry),1998,41(1):45-49. |
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| Authors: | Runan Dong Yong Qiu Shufang Tian Shengqi Wang Xinqi Song |
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| Institution: | (1) State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Department of Chemistry and Department of Chemical Engineering, Tsinghua University, 100084 Beijing, China;(3) Institute of Radiation Medicine, Academy of Military Medical Sciences, 100850 Beijing, China |
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| Abstract: | Photosensitized oxidation of the novel phthalocyanine modified by glycine ZnPc(NHCH\-2COOH)\-4] in the presence of glucose oxidase or plasmid pBR322 DNA was investigated by using gel electrophoresis, capillary electrophoresis and the enzyme activity determination method. As a result, glucose oxidase was cleaved partially by photosensitizer, the cross\|linkage between subunits occurred partially simultaneously and, moreover, enzyme was cleaved continually into pieces. Activity of glucose oxidase was 130 times lower than that of original within 12 h. DNA could be cleaved in the presence of photosensitizer ZnPc(NHCH\-2COOH)\-4. Super helix of DNA was depolymerized into nicked and linear DNA. Then, it was cleaved progressively into pieces. It was suggested that the novel photosensitizer could do apparent damage to biomacromolecules. |
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| Keywords: | phthalocyanine glucose oxidase DNA photosensitized oxidation |
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