| In situ synthesis of DNA micro-arrays using typography technique |
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| 作者单位： | TANG JianXin（State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou 412008, China） ;
HE NongYue（State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou 412008, China;Zhongshan Huoju Polytechnic, Zhongshan 528436, China） ;
LI Song（State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China） ; |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划);湖南省自然科学基金;广东省自然科学基金 |
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| 摘 要： | A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide,which has the celerity,high spatial resolution,lower cost,reliable operation,and high synthetic efficiency.The principle and process of the typography technique for fabricating gene-chips have been described in detail.A suit of poly(terafluoroethylene)devices for synthesizing oligonucleotide arrays were designed and prepared,and the fiber tubes with a number of nano-or micron-channels were em- ployed.The oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography technique.The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms(SNP)and to detect mutations.
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In situ synthesis of DNA micro-arrays using typography technique |
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| Authors: | TANG JianXin HE NongYue LI Song |
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| Abstract: | A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide,which has the celerity,high spatial resolution,lower cost,reliable operation,and high synthetic efficiency.The principle and process of the typography technique for fabricating gene-chips have been described in detail.A suit of poly(terafluoroethylene)devices for synthesizing oligonucleotide arrays were designed and prepared,and the fiber tubes with a number of nano-or micron-channels were em- ployed.The oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography technique.The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms(SNP)and to detect mutations. |
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| Keywords: | typography oligonucleotide in situ synthesis DNA microarrays |
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