| Introducing Bt Gene Into Maize With Ovary Injection |
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| 作者姓名： | 丁群星 谢友菊 戴景瑞 米景九 李太元 田颖川 乔利亚 莽克强 刘宝兰 王音 冯平章 |
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| 作者单位： | Beijing Agricultural University，Beijing 100094，PRC，Beijing Agricultural University，Beijing 100094，PRC，Beijing Agricultural University，Beijing 100094，PRC，Beijing Agricultural University，Beijing 100094，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Plant Protection Institute，Academy of Agricultural Sciences，Beijing 100094，PRC，Plant Protection Institute，Academy of Agricultural Sciences，Beijing 100094，PRC，Plant Protection Institute，Academy of Agricultural Sciences，Beijing 100094，PRC |
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| 摘 要： | It is reported here that Bt toxin gene has been successfully transferred into maize inbred line by ovary injection for the first time both at home and abroad. One transgenic plant (To) has been confirmed by Southern blotting and PCR test, and 71 progenies (T1) from T0 have been obtained through self-pollination. Of these 71 progenies, seven plants demonstrated positive results in the PCR test; four were used to feed Asian corn borer, and certain effect of insect-resistance was observed. The experiments on the ovary injection in Hainan Province have also been repeated, thus providing new chance to the application of genetic engineering to the maize improvement.
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Introducing Bt Gene Into Maize With Ovary Injection |
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| DING Qun-Xing XIE You-Ju,DAI Jing-RuiMI Jing-Jiu.Introducing Bt Gene Into Maize With Ovary Injection[J].Science in China(Chemistry),1994(5). |
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| Authors: | DING Qun-Xing XIE You-Ju DAI Jing-RuiMI Jing-Jiu |
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| Abstract: | It is reported here that Bt toxin gene has been successfully transferred into maize inbred line by ovary injection for the first time both at home and abroad. One transgenic plant (To) has been confirmed by Southern blotting and PCR test, and 71 progenies (T1) from T0 have been obtained through self-pollination. Of these 71 progenies, seven plants demonstrated positive results in the PCR test; four were used to feed Asian corn borer, and certain effect of insect-resistance was observed. The experiments on the ovary injection in Hainan Province have also been repeated, thus providing new chance to the application of genetic engineering to the maize improvement. |
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| Keywords: | maize transformation ovary injection Bt toxin gene |
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