| Engineered Bacterial Fc Receptors |
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| 作者姓名： | 蔡仕英 王园园 姚志建 |
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| 作者单位： | Institute of Basic Medical Sciences，Beijing 100850，PRC，Institute of Basic Medical Sciences，Beijing 100850，PRC，Institute of Basic Medical Sciences，Beijing 100850，PRC |
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| 摘 要： | Five new-type Fc receptor molecules were constructed based on streptococcal protein G (SpG) and staphylococcal protein A (SpA). These protein molecules contain one to six Fc binding domains to immunoglobulins which are structurally different from native SpG or SpA. Their expression levels reached 17-30% of the total bacterial proteins after heat induction in E. colt. Immunodiffusion and ELISA results showed that the engineered protein TG (184 amino acid residues) composed of three SpG C3 domain could bind more broadly and efficiently than the native SpG to the IgGs of human, goat, rabbit, etc. , and its optimal pH for binding became wider (pH5-8) compared with the SpG (pH5) ; and the protein TGA (357AA), fused by protein TG and the A, B, C domains of SpA, displayed both the binding pattern of SpG and SpA.
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Engineered Bacterial Fc Receptors |
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| CAI Shi-Ying WANG Yuan-Yuan and YAO Zhi-Jian.Engineered Bacterial Fc Receptors[J].Science in China(Chemistry),1994(4). |
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| Authors: | CAI Shi-Ying WANG Yuan-Yuan and YAO Zhi-Jian |
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| Abstract: | Five new-type Fc receptor molecules were constructed based on streptococcal protein G (SpG) and staphylococcal protein A (SpA). These protein molecules contain one to six Fc binding domains to immunoglobulins which are structurally different from native SpG or SpA. Their expression levels reached 17-30% of the total bacterial proteins after heat induction in E. colt. Immunodiffusion and ELISA results showed that the engineered protein TG (184 amino acid residues) composed of three SpG C3 domain could bind more broadly and efficiently than the native SpG to the IgGs of human, goat, rabbit, etc. , and its optimal pH for binding became wider (pH5-8) compared with the SpG (pH5) ; and the protein TGA (357AA), fused by protein TG and the A, B, C domains of SpA, displayed both the binding pattern of SpG and SpA. |
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| Keywords: | engineered Fc receptor high level expression binding pattern |
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