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| Effect of Ce~(3+) on spectral characteristic of D1/D2/Cytb559 complex from spinach | |
| 作者姓名： | 洪法水 王雪峰 刘超 苏国兴 宋卫平 吴康 陶冶 赵贵文 |
| 作者单位： | College of Life Sciences，Suzhou University，Suzhou 215006，China，College of Life Sciences，Suzhou University，Suzhou 215006，China，College of Life Sciences，Suzhou University，Suzhou 215006，China，College of Life Sciences，Suzhou University，Suzhou 215006，China，College of Life Sciences，Suzhou University，Suzhou 215006，China，College of Life Sciences，Suzhou University，Suzhou 215006，China，Synchrotron Radiation Laboratory，Institute of High Energy Physics，Chinese Academy of Sciences，Beijing 100039，China，Chemical Department，University of Science and Technology of China，Hefei 230026，China |
| 基金项目： | This work was supported by the National Natural Science Foundation of China (Grant No. 29805003)，Person of Ability Recommended Foundation of Suzhou University (Grant No. XQ316011). |
| 摘 要： | In our early researches, lanthanum and cerium could enter plant and bind to porphyrin of chlorophyll to form Ln3+-chllorophyll. La and Ce greatly increase photosystem II (PSII) activity and PSII electron transport rate, and the fluorescence emission peaks of PSII are blue-shifted 1—4]. Do REEs coordinate with PSII reaction center complex in vivo? Moreover, do REEs coordinate with D1(30 kD)/D2(32 kD)/Cytb559 (~9 kD) reaction center complex of site of producing pri-mary reaction-p… |
Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach |
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| HONG Fashui ,WANG Xuefeng ,LIU Chao ,SU Guoxing ,SONG Weiping ,WU Kang ,TAO Ye & ZHAO Guiwen . College of Life Sciences,Suzhou University,Suzhou ,China, . Synchrotron Radiation Laboratory.Effect of Ce~(3+) on spectral characteristic of D1/D2/Cytb559 complex from spinach[J].Science in China(Chemistry),2003,46(1). | |
| Authors: | HONG Fashui WANG Xuefeng LIU Chao SU Guoxing SONG Weiping WU Kang TAO Ye & ZHAO Guiwen College of Life Sciences Suzhou University Suzhou China Synchrotron Radiation Laboratory |
| Institution: | 1. College of Life Sciences, Suzhou University, Suzhou 215006, China 2. Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100039, China 3. Chemical Department, University of Science and Technology of China, Hefei 230026, China |
| Abstract: | It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D1, D2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+ could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a of UV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD) spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex, and then accelerates the primary reaction of PSII, intensifies function of P680+ primary electron donor of D1/D2/Cytb559, but there is little change in conformation of PSII reaction center complex. |
| Keywords: | Ce3+ spinach D1/D2/Cytb559 complex photosynthesis spectra characteristic |
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