| MOLECULAR CHARACTERIZATION OF RICE Wx GENE |
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| 作者姓名： | 王宗阳 武志亮 邢彦彦 郑霏琴 郭小丽 张伟国 洪孟民 |
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| 作者单位： | Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC |
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| 基金项目： | Project supported by the grants from the High Technology Development Programme of China and the Rockefeller Foundation. |
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| 摘 要： | The complete nucleotide (nt) sequence of the rice waxy(Wx) gene, which is responsible for the synthesis of amylose in endosperm and pollen, has been determined by a combination of restriction mapping and nt sequence analysis of two overlapping genomic DNA clones. The entire gene is about 5.5 kb in length. The alignment of the nt sequence of the Wx gene from rice with those of maize (Klsgen, R. B. et al.) and barley (Rohde, W. et al.) revealed the presence of thirteen introns and fourteen exons. The full-length of Wx protein in cluding transit peptide is 609 amino acid (aa) residues. The calculated molecular weight of rice Wx preprotein is about 72 kD. There is no significant difference between the similarity scores of the aa sequence deduced from the rice Wx gene compared with those of maize and barley. However, the nt sequences of the 5'-end upstream, 3'-end downstream and introns of the rice Wx gene, as well as the aa sequence of the transit peptide region of the Wx preprotein have low similarity scor
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MOLECULAR CHARACTERIZATION OF RICE Wx GENE |
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| WANG ZONG-YANG WU ZHI-LIANG XING YAN-YAN ZHENG FEI-OIN GUO XIAO-LJ ZHANG WEI-GUO AND HONG MENG-MIN.MOLECULAR CHARACTERIZATION OF RICE Wx GENE[J].Science in China(Chemistry),1992(5). |
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| Authors: | WANG ZONG-YANG WU ZHI-LIANG XING YAN-YAN ZHENG FEI-OIN GUO XIAO-LJ ZHANG WEI-GUO AND HONG MENG-MIN |
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| Abstract: | The complete nucleotide (nt) sequence of the rice waxy(Wx) gene, which is responsible for the synthesis of amylose in endosperm and pollen, has been determined by a combination of restriction mapping and nt sequence analysis of two overlapping genomic DNA clones. The entire gene is about 5.5 kb in length. The alignment of the nt sequence of the Wx gene from rice with those of maize (Klsgen, R. B. et al.) and barley (Rohde, W. et al.) revealed the presence of thirteen introns and fourteen exons. The full-length of Wx protein in cluding transit peptide is 609 amino acid (aa) residues. The calculated molecular weight of rice Wx preprotein is about 72 kD. There is no significant difference between the similarity scores of the aa sequence deduced from the rice Wx gene compared with those of maize and barley. However, the nt sequences of the 5'-end upstream, 3'-end downstream and introns of the rice Wx gene, as well as the aa sequence of the transit peptide region of the Wx preprotein have low similarity scores in comparison with those of maize and barley. |
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| Keywords: | Wx gene rice amylose DNA sequence |
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