| PARADOXICAL EFFECT OF A GnRH AGONIST ON STEROIDOGENESIS IN CULTURED MONKEY GRANULOSA CELLS |
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| 作者姓名： | 刘以训 胡召元 冯强 邹如金 |
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| 作者单位： | Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080，PRC，Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080，PRC，Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080，PRC，Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080，PRC Kunming Institute of Zoology，Kunming，China. |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China and Rockefeller Foundation, New York. |
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| 摘 要： | In this study we demonstrate: (i) The GnRH agonist exerts a direct dose-dependet stimulative effect on the aromatase activity and progesterone production in cultured monkey granulosa cells; (ii)the stimulative effect on steroidogenesis can be completely blocked by concomitant treatment with a GnRH antagonist, suggesting that the actions of GnRH are mediated through stringent stereospecific recongnition sites; (iii) in addition to the stimulative effect, the GnRH agonist in the presence of gonadotropins also exerts an inhibitory effect, even though the peptide by itself is more effective in the stimulation of steroidogenesis, and the stimulation of gonadotropin on steroidogenesis could be gradually restored by decreasing the concentration of the GnRH agonist in the culture; and (iv) paradoxical effect can also be observed in the presence of cAMP-inducing agents, suggesting that the inhibitory action of the peptide on gonadotropin-induced steroidogenesis is localized at a step distal to the stringent reco
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PARADOXICAL EFFECT OF A GnRH AGONIST ON STEROIDOGENESIS IN CULTURED MONKEY GRANULOSA CELLS |
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| LIU YI-XUN HU ZHAO-YUAN FENG QIANGAND ZOU RU-JIN.PARADOXICAL EFFECT OF A GnRH AGONIST ON STEROIDOGENESIS IN CULTURED MONKEY GRANULOSA CELLS[J].Science in China(Chemistry),1991(12). |
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| Authors: | LIU YI-XUN HU ZHAO-YUAN FENG QIANGAND ZOU RU-JIN |
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| Abstract: | In this study we demonstrate: (i) The GnRH agonist exerts a direct dose-dependet stimulative effect on the aromatase activity and progesterone production in cultured monkey granulosa cells; (ii)the stimulative effect on steroidogenesis can be completely blocked by concomitant treatment with a GnRH antagonist, suggesting that the actions of GnRH are mediated through stringent stereospecific recongnition sites; (iii) in addition to the stimulative effect, the GnRH agonist in the presence of gonadotropins also exerts an inhibitory effect, even though the peptide by itself is more effective in the stimulation of steroidogenesis, and the stimulation of gonadotropin on steroidogenesis could be gradually restored by decreasing the concentration of the GnRH agonist in the culture; and (iv) paradoxical effect can also be observed in the presence of cAMP-inducing agents, suggesting that the inhibitory action of the peptide on gonadotropin-induced steroidogenesis is localized at a step distal to the stringent recognition sites. |
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| Keywords: | GnRH agonist rhesus monkey granulosa cells paradoxical effect steroidogenesis |
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