| Langmuir-Blodgett films of phycobiliproteins (I)——Monomolecular film and conformational studies of R-phycoerythrin |
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| 引用本文： | 何靳安,蒋丽金,江龙,毕只初,李津如.Langmuir-Blodgett films of phycobiliproteins (I)——Monomolecular film and conformational studies of R-phycoerythrin[J].中国科学B辑(英文版),1996(3). |
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| 作者姓名： | 何靳安 蒋丽金 江龙 毕只初 李津如 |
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| 作者单位： | Institute of Photographic Chemistry，Chinese Academy of Sciences，Beijing 100101，China，Institute of Photographic Chemistry，Chinese Academy of Sciences，Beijing 100101，China，Institute of Photographic Chemistry，Chinese Academy of Sciences，Beijing 100101，China，Institute of Photographic Chemistry，Chinese Academy of Sciences，Beijing 100101，China，Institute of Photographic Chemistry，Chinese Academy of Sciences，Beijing 100101，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China |
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| 摘 要： | The surface pressure-area (π-A) isotherm of R-phycoerythrin (R-PE) at the air-water interface has been measured. The results indicate that R-PE can form the monomolecular film. Moreover, the molecule-occupied area extrapolating the linear part of the n-A isotherm is identical with that when an R-PE molecule is located at the interface with its disk plane parallel to the air-water interface. The transmission electron micrograph (TEM) and the measurement of the thickness of the protein monolayer by ellipsometry show that the orientation of R-PE disk plane on the substrate is parallel to the plane of substrate. Absorption and fluorescence spectra of R-PE LB multilayers were obtained through transferring R-PE monolayer at the air-water interface to the substrates at the proper surface pressure by Langmuir-Blodgett (LB) technique. These spectra of R-PE LB films do not show distinct differences from those in aqueous solution. Comparative studies of circular dichroism (CD) spectra of the protein between in aq
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Langmuir-Blodgett films of phycobiliproteins (I) --Monomolecular film and conformational studies of R-phycoerythrin |
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| HE Jinan JIANG Lijin JIANG LongBI Zhichu and LI Jinru.Langmuir-Blodgett films of phycobiliproteins (I) --Monomolecular film and conformational studies of R-phycoerythrin[J].Science in China(Chemistry),1996(3). |
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| Authors: | HE Jinan JIANG Lijin JIANG LongBI Zhichu and LI Jinru |
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| Abstract: | |
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| Keywords: | R-phycoerythrin monolayer ellipsometry LB technique TEM conformation of protein |
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