| 界面电场对血红蛋白在多孔金电极上的吸附和生物活性的影响(英文) |
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| 引用本文： | 王翠红,孔逸冰,夏兴华.界面电场对血红蛋白在多孔金电极上的吸附和生物活性的影响(英文)[J].电化学,2011,17(1):24-30. |
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| 作者姓名： | 王翠红 孔逸冰 夏兴华 |
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| 作者单位： | 南京大学生命分析化学教育部重点实验室化学化工学院; |
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| 基金项目： | supported by grants from the National Natural Science Foundation of China(NSFC:20828006,21035002); the National Science Fund for Creative Research Groups(20821063) |
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| 摘 要： | 蛋白质的界面吸附及其生物活性因它在构建生物传感、生物电子器件和生物燃料电池等方面具有重要的作用而倍受关注.对此,界面电场是吸附的一个重要影响因素,它能明显地影响蛋白质分子在材料界面的吸附量、分子构象以及分子定向.本文应用电化学方法和红外光谱技术研究了血红蛋白在三维多孔金膜电极上的吸附动力学及其生物活性随界面电场的变化关系.结果表明,由界面电场产生的过量表面电荷可借助与蛋白质分子之间的静电作用加速蛋白质分子在电极表面的吸附,提高其吸附量;但是,过高的界面电场将破坏吸附蛋白质的构象以及降低它还原过氧化氢的催化活性;只有在零电荷电位下,吸附在电极表面的血红蛋白才能保持其天然的构象和生物催化活性.本研究将为生物传感器、生物电子器件和生物燃料电池的构建提供理论依据,加深对荷电生物界面上生物分子界面行为的认识.
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| 关 键 词： | 吸附 蛋白质 血红蛋白 荷电界面 界面电场 生物催化活性 |
| 收稿时间： | 2011-02-28 |
Influence of Electric Field on the Adsorption and Bioactivity of Hemoglobin on A Macroporous Gold Electrode |
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| WANG Cui-hong,KONG Yi-bing,XIA Xing-hua.Influence of Electric Field on the Adsorption and Bioactivity of Hemoglobin on A Macroporous Gold Electrode[J].Electrochemistry,2011,17(1):24-30. |
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| Authors: | WANG Cui-hong KONG Yi-bing XIA Xing-hua |
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| Institution: | WANG Cui-hong,KONG Yi-bing,XIA Xing-hua(Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,China) |
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| Abstract: | The adsorption and bioactivity of proteins at interfaces have been widely studied due to their important role in the construction of biosensors,bioelectronics and biofuel cells.Interfacial electric field is one of the important factors which could affect the adsorption and bioactivity of proteins at materials surfaces.It could dramatically change the adsorption density,molecular conformation and orientation at material surfaces.In this paper,the influence of interfacial electric field on the adsorption kine... |
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| Keywords: | adsorption protein hemoglobin charged surface electric field biocatalytic activity |
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