Transferase and hydrolase activities of phospholipase D from the roots ofAconitum arcuatum

The phospholipase D fromAconitum arcuatum has been studied. The optimum pH values have been determined: 6.3 for the hydrolase activity and 8.0 for the transferase activity. Calcium ions activate the phospholipase D: the hydrolase activity most strongly at 8 and 30 µmole and the transferase activity at 30 µmole. The hydrolase activity of the phospholipase D is not activated by low concentrations of sodium dodecyl sulfate, while the transferase activity is activated to a considerable degree. The results obtained are compared with those on the activation of cabbage phospholipase D.