A sensitive chromatographic determination of hydrazines by naphthalene-2,3-dialdehyde derivatization |
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| Authors: | Alexander D Smolenkov Alla V Chernobrovkina Roman S Smirnov Mikhail G Chernobrovkin Oleg A Shpigun |
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| Institution: | 1. Department of Chemistry , Lomonosov Moscow State University , Leninskie Gory, 1/3, GSP-1, Moscow 119991 , Russia smolenkov@analyt.chem.msu.ru;3. Department of Chemistry , Lomonosov Moscow State University , Leninskie Gory, 1/3, GSP-1, Moscow 119991 , Russia;4. Drugs Technology LTD , 2?A, Build. 1 Rabochaya Str., Khimki, Moscow District , 141400, Russia |
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| Abstract: | A new high-performance liquid chromatography (HPLC) method for the sensitive simultaneous determination of hydrazine (Hy), monomethylhydrazine (MMH) and 1,1-dimethylhydrazine (UDMH) based upon the derivatization of hydrazines with naphthalene-2,3-dialdehyde and the separation of the derivatives on Zorbax Eclipse AAA column in a single chromatographic run under acidic conditions (pH 2.4) was developed. Hydrazine and monomethylhydrazine derivatives were found to be strongly fluorescent at λex?=?273?nm, λem?=?500?nm. It was shown that UDMH derivative can be detected as non-fluorescent hydrazone at 290?nm by UV-detection. Limits of detection were 0.05?µg?·?L?1 for Hy and MMH, and 1?µg?·?L?1 for UDMH for the injection volume of 100?µL. The method was validated for water sample analysis. It proved to be selective, accurate and precise with the supplementary advantage of the simple and rapid sample preparation. |
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| Keywords: | liquid chromatography pre-column derivatization hydrazines naphthalene-2 3-dialdehyde |
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