Comparison of BOD optical fiber biosensors based on different microorganisms immobilized in ormosil matrixes |
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| Authors: | Yuan-Jing Dai Ling Lin Pei-Wei Li Xiao-Ru Wang Kwok-Yin Wong |
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| Institution: | 1. The Key Laboratory of Analytical Sciences of Ministry of Education and Department of Chemistry , Xiamen University , Xiamen 361005, China;2. The Key Laboratory of Analytical Sciences of Ministry of Education and Department of Chemistry , Xiamen University , Xiamen 361005, China;3. Department of Biology , Indiana University , Bloomingtion, IN, 47401, USA;4. Department of Applied Biology and Chemical technology , The Hong Kong Polytechnic University , Hunghom, Kowloon, Hong Kong, China |
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| Abstract: | Fiber-optical microbial sensors for determination of biochemical oxygen demand (BOD) are described. Sensing films consisting of layers of an oxygen-sensitive fluorescent material and two different kinds of seawater microorganisms immobilized in poly(vinyl alcohol) sol–gel matrix were investigated. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) perchlorate was used as the oxygen fluorescent quenching indicator. After preconditioning, the BOD biosensors could consistently perform well for up to one month. For films of domestic bacilli and films of sieved bacteria from seawater, the linear fluctuant coefficients (R 2) in the range of 4–200?mg/L were 0.9975 and 0.9783 when a glucose/glutamate BOD standard was applied. The relative error of standard deviations for the two microorganism-immobilized BOD sensing films were within 4%?and 2%?of the mean value, respectively. The effects of temperature, pH and sodium chloride concentration on the two microbial films were also studied. For low biochemical oxygen demand, a film of sieved bacteria from seawater had superior sensitivity and is expected to be developed further. |
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| Keywords: | BOD biosensor Microorganisms Sol–gel Ormosils |
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