| 荧光猝灭法测定超氧阴离子自由基的研究 |
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| 引用本文： | 蒲法章,马淑慧,韦继超,王丹丹,高吉刚,周杰.荧光猝灭法测定超氧阴离子自由基的研究[J].分析科学学报,2009,25(5). |
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| 作者姓名： | 蒲法章 马淑慧 韦继超 王丹丹 高吉刚 周杰 |
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| 作者单位： | 山东农业大学化学与材料科学学院,山东泰安,271018 |
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| 摘 要： | 合成了一种新的荧光探针试剂香草醛缩苯胺,利用元素分析、红外光谱等手段对探针试剂进行结构表征;结合邻苯三酚的自氧化作用,建立了一种荧光法测定超氧阴离子自由基(O_2~(-·))的新方法.该方法具有操作简单、灵敏度高和选择性好等特点.邻苯三酚线性范围为4.0×10~(-6)～1.0×10~(-5) mol·~(-1).检出限为2.0×10~(-7) mol·~(-1).方法用于大蒜等样品中超氧化物歧化酶(SOD)活性检测,结果满意.
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| 关 键 词： | 超氧阴离子自由基(O- ·2) 香草醛缩苯胺 荧光探针 荧光法 |
The Study on Fluorescence Quenching for Determination of Superoxide Radical |
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| PU Fa-zhang,MA Shu-hui,WEI Ji-chao,WANG Dan-dan,GAO Ji-gang,ZHOU Jie.The Study on Fluorescence Quenching for Determination of Superoxide Radical[J].Journal of Analytical Science,2009,25(5). |
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| Authors: | PU Fa-zhang MA Shu-hui WEI Ji-chao WANG Dan-dan GAO Ji-gang ZHOU Jie |
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| Institution: | PU Fa-zhang,MA Shu-hui,WEI Ji-chao,WANG Dan-dan,GAO Ji-gang,ZHOU Jie(College of Chemistry and Material Science,Shandong Agricultural University,Tai'an 271018) |
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| Abstract: | A new fluorescent reagent N-3-methoxy-4-hydroxyphenylmenthylidene]-aniline was synthesized, and its structural characters was characterized by elemental analysis and infrared spectroscopy. With the new synthesized fluorescence reagent, a novel fluorescence quenching method for determination of superoxide radical was developed by coupling the reaction of pyrogallic auto-oxidation reaction. The method is simple and sensitive. The linear range and detection limit are 4.0×10~(-6)～1.0×10~(-5) mol·~(-1) and 2.0×10~(-7) mol·~(-1), respectively. The approach has been applied to determine superoxide radical in garlic sample with satisfying results. |
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| Keywords: | N-[3-methoxy-4-hydroxyphenylmenthylidene]-aniline Superoxide radical Fluorescence quenching Garlic |
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