| 毒素蛋白ColicinE1与磷脂膜相互作用的荧光光谱研究临界摩尔比的测定 |
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| 引用本文： | 武轶,隋森芳,李庆喜,王敬尊.毒素蛋白ColicinE1与磷脂膜相互作用的荧光光谱研究临界摩尔比的测定[J].分析科学学报,1996(3). |
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| 作者姓名： | 武轶 隋森芳 李庆喜 王敬尊 |
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| 作者单位： | 清华大学生物科学与技术系，北京微量化学研究所 |
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| 基金项目： | 国家自然科学基金,攀登计划资助项目 |
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| 摘 要： | 磷脂－蛋白相互作用的临界摩尔比是研究膜脂－蛋白相互作用的重要参数．本文利用荧光光谱技术首次测定了毒素蛋白ＣｏｌｉｃｉｎＥ１在不同条件下与不同磷脂膜相互作用的临界摩尔比并通过临界摩尔比的变化讨论了插膜蛋白与磷脂膜相互作用的规律，为进一步探讨毒素蛋白的插膜机制提供了重要的基础
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| 关 键 词： | ColicinE1 临界摩尔比 脂质体 荧光 光谱 |
Studies of Interaction of Colicin E 1 with Phospholipid Vesicles by Fluorescence Spectroscopy The Measurement of Critical Molar Ratio Lipid to Protein |
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| Wu Yi,Sui Senfang.Studies of Interaction of Colicin E 1 with Phospholipid Vesicles by Fluorescence Spectroscopy The Measurement of Critical Molar Ratio Lipid to Protein[J].Journal of Analytical Science,1996(3). |
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| Authors: | Wu Yi Sui Senfang |
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| Abstract: | The critical molar ratio for interaction between lipid and protein is an important parameter in the studies of interaction between lipid and protein. In this paper we measured the critical molar ratio for the interaction of Colicin E 1 with different lipid vesicles using fluorescence spectroscopy under different conditions. According to the changes of critical molar ratio the law for the interaction of pore forming protein with lipids was discussed, providing some basic data for the research of mechnism of membrane insertion. |
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| Keywords: | Colicin E 1 Critical molar ratio Vesicle Fluorescence spectroscopy |
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