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DNA分子结构的多态性研究 (Ⅱ) 吖啶橙凝聚体变化诱导的质粒DNA大分子构象的研究
引用本文:冯喜增,林璋,杨林静,李清,王琛,白春礼.DNA分子结构的多态性研究 (Ⅱ) 吖啶橙凝聚体变化诱导的质粒DNA大分子构象的研究[J].分析科学学报,1999(3).
作者姓名:冯喜增  林璋  杨林静  李清  王琛  白春礼
作者单位:中国科学院化学研究所
基金项目:中国科学院“九五”重大科研项目基金,中国博士后科学基金
摘    要:利用吸收光谱和荧光光谱方法,研究了吖啶橙(AO)与质粒DNA水溶液、以及含胶束介质的吖啶橙与质粒DNA溶液体系的相互结合作用及减色效应。结果表明:吖啶橙对质粒DNA的吸收光谱有减色效应;含十二烷基硫酸钠(SDS)的AO水溶液体系中,随着SDS浓度的增加,其光谱结果表现为由凝聚态向单体的转化。而在含十二烷基硫酸钠(SDS)的AO与质粒DNA溶液体系中,吖啶橙凝聚态随SDS浓度的增加,对AO与质粒DNA相互结合产生协同的减色效应,使质粒DNA空间结构发生缩拢。进一步采用电泳法研究了AO凝聚态可能对质粒DNA构象的影响,结果表明:在AO与质粒DNA溶液体系中,AO浓度的增加对质粒DNA构象未产生影响;而在含有SDS的AO与质粒DNA的溶液体系中,由于SDS对AO凝聚态的解聚作用,以及SDS对质粒DNA减色效应的协同作用,使得质粒DNA的构象发生变化,诱导质粒DNA形成超螺旋构象

关 键 词:吖啶橙凝聚体  胶束介质  减色效应  质粒DNA超螺旋构象

The Investigation of the Various Structures of DNA \= Molecules() Conformation of Plasmid DNA Induced \= by Acridine Orange Aggregates
Feng Xizeng,Lin Zhan,Yang Linjing,Li Qing,Wang Chen,Bai Chunli\.The Investigation of the Various Structures of DNA \= Molecules() Conformation of Plasmid DNA Induced \= by Acridine Orange Aggregates[J].Journal of Analytical Science,1999(3).
Authors:Feng Xizeng  Lin Zhan  Yang Linjing  Li Qing  Wang Chen  Bai Chunli\
Institution:Feng Xizeng,Lin Zhan,Yang Linjing,Li Qing,Wang Chen,Bai Chunli\+ *
Abstract:The binding interaction of acridine orange (AO) with plasmid DNA and the DNA hypochromic effect with and without micelle medium in the solution were investigated by absorption spectra and fluorescence spectra. The results showed that AO has hypochromic effect on DNA absorption spectra. With the increasing of sodium dodecyl sulfate (SDS) concentration in AO solution, the spectra shows the AO can change from aggregaed state to monomer. However, in the solution of plasmid DNA and AO, the increment of SDS concentration has a concerted hypochromic effect on the interaction between AO and plasmid DNA, which makes DNA contract. We further studied the possible effect of AO aggregates on plasmid DNA conformation. It appears that the increment of AO concentration has no much influence on DNA conformation in the AO and plasmid DNA solution. However, in the solution of AO and DNA with SDS,SDS has disaggregation effect on AO and concerted influence on plasmid DNA hypochromic effect, which changes the DNA co nformation and induces plasmid DNA into superhelix conformation.\=
Keywords:AO aggregation  Micelle medium  Hypochromic effect      Superhelix conformation of plasmid DNA  
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