| 固定化脂肪酶活性的X射线微区分析 |
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| 引用本文： | 丁良,姚子华.固定化脂肪酶活性的X射线微区分析[J].分析科学学报,2003,19(2):160-162. |
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| 作者姓名： | 丁良 姚子华 |
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| 作者单位： | 1. 河北大学理化分析中心;河北省职工医学院,保定,071002
2. 河北大学理化分析中心 |
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| 摘 要： | 利用X射线微区分析的方法，对吸附交联法得到的固定化脂肪酶的微观活性进行了分析。结果表明：以合成出的大孔吸附树脂为固定化酶栽体，酶活较高，活性脂肪酶分布较均匀。并得到了固定化脂肪酶的活性定位的最佳条件。
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| 关 键 词： | 固定化脂肪酶 酶活性 X射线微区分析 大孔吸附树脂 |
| 文章编号： | 1006-6144(2003)02-0160-03 |
| 修稿时间： | 2002年8月27日 |
X-ray Microanalysis of the Activity of Immobilized Lipaes |
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| Abstract: | The localization of activity of immobilzed lipaes was studied by X ray microanalysis. Olive oil and CaCl 2 was used as substrate and capture, respectively. Substrate was catalysed by immobilized lipaes to produce fatty acid and fatty acid was captured by CaCl 2 to form precipitate MCOOCa. Precipitation deposited active site of immobilized lipaes. The results showed that using the macroporous resins to immobilize lipaes, the enzyme activity was high, and distribution of activated enzyme was uniform. The optimum condition of localization of activity of immobilized lipaes was studied. |
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| Keywords: | X ray microanalysis Immobilized lipase Macroporous resins Enzyme activity |
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