| 柱前衍生高效液相色谱测定小儿复方氨基酸注射液中氨基酸 |
| |
| 引用本文： | 朱影恬,曾雯瑜,张中伟,罗珍,王远兴.柱前衍生高效液相色谱测定小儿复方氨基酸注射液中氨基酸[J].分析科学学报,2009,25(6). |
| |
| 作者姓名： | 朱影恬 曾雯瑜 张中伟 罗珍 王远兴 |
| |
| 作者单位： | 南昌大学食品科学与技术国家重点实验室,江西南昌,330047 |
| |
| 摘 要： | 建立了高效液相色谱(HPLC)测定小儿复方氨基酸注射液中各种氨基酸含量的方法.采用Kromasil C18(250×4.6 mm,5 μm)色谱柱,以2,4-二硝基氟苯为衍生试剂,梯度洗脱.流动相A为乙腈∶水(50∶50,V/V),流动相B为0.04 mol/L的磷酸二氢钾溶液(pH=6.8);流速为1.0 mL/min;检测波长为360 nm.所测氨基酸的线性范围为0.008～0.2213 mg/mL,平均回收率在97.85%～102.86%之间.
|
| 关 键 词： | 高效液相色谱法 柱前衍生 2 4-二硝基氟苯 氨基酸 |
Determination of Amino Acids in Children Compound Amino Acid Injection by Pre-column Derivatization High Performance Liquid Chromatography |
| |
| ZHU Ying-tian,ZENG Wen-yu,ZHANG Zhong-wei,LUO Zhen,WANG Yuan-xing.Determination of Amino Acids in Children Compound Amino Acid Injection by Pre-column Derivatization High Performance Liquid Chromatography[J].Journal of Analytical Science,2009,25(6). |
| |
| Authors: | ZHU Ying-tian ZENG Wen-yu ZHANG Zhong-wei LUO Zhen WANG Yuan-xing |
| |
| Institution: | ZHU Ying-tian,ZENG Wen-yu,ZHANG Zhong-wei,LUO Zhen,WANG Yuan-xing*(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047) |
| |
| Abstract: | A pre-column derivatization high performance liquid chromatogrphy method for the determination of amino acids in the children compound amino acid injection was established. The sample was derivatized with 2,4-dinitrofluorobenzene(DNFB). Kromasil C18 column (250×4.6 mm,5 μm) was used. The gradient elution was performed using mixed solution of acetonitrile and water (50∶50,V/V), and 0.04 mol/L KH_2PO_4(pH=6.8) as the mobile phases respectively at a flow rate of 1.0 mL/min. All the amino acids measured show good linear relationship within 0.008～0.2213 mg/mL and average recoveries between 97.85% and 102.86%. |
| |
| Keywords: | High performance liquid chromatography Pre-column derivatization 2 4-dimitroflnorobenzene Amino acids |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
