| 二甲酚橙-KBrO_3体系阻抑反应动力学光度法测定蛋白质 |
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| 引用本文： | 孙晓艳,李贤珍,李建平.二甲酚橙-KBrO_3体系阻抑反应动力学光度法测定蛋白质[J].分析科学学报,2009,25(5). |
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| 作者姓名： | 孙晓艳 李贤珍 李建平 |
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| 作者单位： | 1. 中国地质科学院郑州矿产综合利用研究所检测中心,河南郑州,450006
2. 桂林工学院材料与化学工程系,广西桂林,541004 |
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| 摘 要： | 在稀硫酸介质中,蛋白质可灵敏地阻抑溴酸钾氧化二甲酚橙褪色的兰多尔特(Landolt)反应,据此建立了阻抑动力学光度法测定痕量蛋白质的新方法.在优化实验条件下,于430 nm波长处进行测定,蛋白质含量在0.2～2.0 mg/L范围内与吸光度呈线性关系,检出限为0.141 mg/L,标准加入回收结果满意.
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| 关 键 词： | 蛋白质 阻抑反应 动力学光度法 兰多尔特反应 |
Determination of Trace BSA by Inhibition Kinetic Spectrophotometry |
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| SUN Xiao-yan,LI Xian-zhen,LI Jian-ping.Determination of Trace BSA by Inhibition Kinetic Spectrophotometry[J].Journal of Analytical Science,2009,25(5). |
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| Authors: | SUN Xiao-yan LI Xian-zhen LI Jian-ping |
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| Institution: | SUN Xiao-yan1,LI Xian-zhen1,LI Jian-ping2(1.Zhengzhou Institute of Multipurpose Utilization of Mineral Resources CAGS,Zhengzhou 450006,2.Depatment of Materials and Chemistry Engineering,Guilin University of Technology,Guilin 541004) |
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| Abstract: | In this paper, the discoloration of Xylenol orange by potassium bromate in H_2SO_4 medium-Landolt reaction was studied.Based on this fact, a new method for the determination of trace BSA was established by inhibition kinetic spectrophotometry.Under the optimum condition, the linear range is 0.2～2.0 μg/mL, and the detection limit of the method was 0.141 mg/L.The developed method was applied to the determination of BSA with satisfactory results. |
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| Keywords: | Protein Inhibition Kinetic spectrophotometry Landolt reaction |
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