| 高效液相色谱法测定银杏果中有机酸的含量 |
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| 引用本文： | 陈向明,代现平,董秀丽.高效液相色谱法测定银杏果中有机酸的含量[J].分析科学学报,2011,27(3):336-338. |
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| 作者姓名： | 陈向明 代现平 董秀丽 |
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| 作者单位： | 滨州医学院药学院; |
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| 基金项目： | 国家自然科学基金(No.30970298) |
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| 摘 要： | 建立了反相高效液相色谱测定银杏果中有机酸含量的方法.方法采用ultimate-C18色谱柱,以pH=2.0的磷酸盐缓冲溶液为流动相,流速0.5 mL/min,检测波长210 nm,在22 min内实现了7种有机酸的基线分离.有机酸的检出限在0.001～0.067μg/mL之间.所建立的方法具有分析速度快、线性范围宽、灵...
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| 关 键 词： | 高效液相色谱 有机酸 银杏果 |
Determination of Organic Acids in Ginkgo Fruit by High Performance Liquid Chromatography |
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| CHEN Xiang-ming,DAI Xian-ping,DONG Xiu-li.Determination of Organic Acids in Ginkgo Fruit by High Performance Liquid Chromatography[J].Journal of Analytical Science,2011,27(3):336-338. |
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| Authors: | CHEN Xiang-ming DAI Xian-ping DONG Xiu-li |
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| Institution: | CHEN Xiang-ming*,DAI Xian-ping,DONG Xiu-li(College of Pharmacy,Binzhou Medical University,Yantai 264003) |
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| Abstract: | A quantitative reversed-phase high performance liquid chromatography method using ultraviolet detector has been developed for the determination of seven organic acids.A ultimate-C18 column was used with phosphate buffer solution(pH=2.0) as the mobile phase at a flow rate of 0.5 mL/min.The seven organic acids were separated in 22 min and the detection limits were between 0.001~0.067 mg/L.The results indicate that this method is fast,sensitive,reproducible and practical for the routine analysis of organic aci... |
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| Keywords: | High performance liquid chromatography Organic acids Ginkgo fruit |
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