| 柱前衍生-反相高效液相色谱测定牛磺罗定及固体制剂含量 |
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| 引用本文： | 陈曦,雷勇,张宁,王业秋,逢居龙.柱前衍生-反相高效液相色谱测定牛磺罗定及固体制剂含量[J].分析科学学报,2011,27(3):339-342. |
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| 作者姓名： | 陈曦 雷勇 张宁 王业秋 逢居龙 |
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| 作者单位： | 中国医学科学院药用植物研究所;黑龙江中医药大学; |
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| 摘 要： | 本文建立了对甲苯磺酰氯柱前衍生-反相高效液相色谱紫外检测法测定牛磺罗定及其固体制剂含量的方法.利用牛磺罗定中含有仲胺基的特点,以对甲基苯磺酰氯柱前衍生牛磺罗定,Venusil XBP C18色谱柱分离,在240 nm波长检测,线性范围为0.016～0.160 g·L-1(r=0.9995),衍生化产物在24 h内稳定,...
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| 关 键 词： | 柱前衍生化 高效液相色谱 对甲基苯磺酰氯 牛磺罗定 |
Determination of Taurolidine by Pre-column Derivatization and Reversed-Phase High Performance Liquid Chromatography |
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| CHEN Xi,LEI Yong,ZHANG Ning,WANG Ye-qiu,PANG Ju-long.Determination of Taurolidine by Pre-column Derivatization and Reversed-Phase High Performance Liquid Chromatography[J].Journal of Analytical Science,2011,27(3):339-342. |
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| Authors: | CHEN Xi LEI Yong ZHANG Ning WANG Ye-qiu PANG Ju-long |
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| Institution: | CHEN Xi1,LEI Yong2,ZHANG Ning*2,WANG Ye-qiu2,PANG Ju-long2(1.Chinese Academy of Medical Sciences Institute of Medicinal Plants,Beijing 100193,2.Heilongjiang University of Chinese Medicine,Harbin 150040) |
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| Abstract: | A method using pre-column derivatization and reserved-phase high performance liquid chromatography(RP-HPLC) UV detection was developed to determine taurolidine.Taurolidine was derivated with p-toluenesulfonyl chloride and then separated by Hypersil ODS C18 column,and detected under the 240 nm wavelength.The linear range of taurolidine was from 0.016 to 0.160 g/L with the recoveries from 98.5% to 101.2%.Derivative products were relatively stable during 24 h(RSD=3.8%).The method was simple,convenient,sensitiv... |
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| Keywords: | Pre-column derivatization High performance liquid chromatography p-toluenesulfonyl chloride Taurolidine UV detection |
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