| 柱前衍生高效液相色谱法分析海带岩藻聚糖的单糖及糖醛酸组成 |
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| 引用本文： | 王泽文,冷凯良,孙伟红,翟毓秀,邢丽红,苗钧魁.柱前衍生高效液相色谱法分析海带岩藻聚糖的单糖及糖醛酸组成[J].分析科学学报,2011,27(1):26-30. |
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| 作者姓名： | 王泽文 冷凯良 孙伟红 翟毓秀 邢丽红 苗钧魁 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所;中国海洋大学食品科学与工程学院; |
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| 基金项目： | 国家科技支撑计划(No.2007BAD62B03); 基础科研业务费(2007-gy-02); 农业部标准化项目 |
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| 摘 要： | 建立了一种柱前衍生高效液相色谱法同时测定海带中岩藻聚糖的单糖及糖醛酸组成的方法.岩藻聚糖经4 mol/L三氟乙酸降解后,用1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化,并采用配有紫外检测器的反相高效液相色谱仪在250 nm波长下进行测定,实现了7种单糖和糖醛酸的良好分离.结果表明:该岩藻聚糖样品由5种单糖和2种...
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| 关 键 词： | 岩藻聚糖 1-苯基-3-甲基-5-吡唑啉酮 柱前衍生化 高效液相色谱法 |
Analysis of Monosaccharides and Uronic Acids in Fucoidon from Seaweed by Pre-column Derivatization High Performance Liquid Chromatography |
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| WANG Ze-wen,LENG Kai-liang,SUN Wei-hong,ZHAI Yu-xiu,XING Li-hong,MIAO Jun-kui.Analysis of Monosaccharides and Uronic Acids in Fucoidon from Seaweed by Pre-column Derivatization High Performance Liquid Chromatography[J].Journal of Analytical Science,2011,27(1):26-30. |
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| Authors: | WANG Ze-wen LENG Kai-liang SUN Wei-hong ZHAI Yu-xiu XING Li-hong MIAO Jun-kui |
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| Institution: | WANG Ze-wen1,2,LENG Kai-liang1,SUN Wei-hong1,ZHAI Yu-xiu1,XING Li-hong1,MIAO Jun-kui1(1.Yellow Sea Fisheries Research Institute Academy of Fishery Sciences,Qingdao 266071,2.Department of food science and engineering,Ocean University of China,Qingdao 266100) |
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| Abstract: | High performance liquid chromatography(HPLC) was employed for the analysis of monosaccharide and uronic acids in fucoidon from fresh seaweed with pre-column derivatization.After degradated by 4 mol/L trifluoroacetic acid and derivatived with 1-phenyl-3-methyl-5-pyrazolone(PMP),monosaccharides and uronic acids in fucoidon were separated by reversed-phase HPLC with gradient elution,and monitored by ultraviolet detector at 250 nm.The result show that fucoidon consists of 5 kinds of monosaccharides and 2 kinds ... |
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| Keywords: | Fucoidon 1-phenyl-3-methyl-5-pyrazolone Pre-column derivatization High performance liquid chromatography |
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