| 荧光猝灭法研究氟比洛芬与DNA相互作用 |
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| 引用本文： | 王晓霞,敖登高娃,阿娟.荧光猝灭法研究氟比洛芬与DNA相互作用[J].分析科学学报,2009,25(6). |
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| 作者姓名： | 王晓霞 敖登高娃 阿娟 |
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| 作者单位： | 1. 内蒙古大学化学化工学院,内蒙古呼和浩特,010021
2. 内蒙古农业大学理学院,内蒙古呼和浩特,010019 |
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| 基金项目： | 内蒙古教育厅基金，内蒙古教育厅项目，内蒙古大学研究生创新基金 |
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| 摘 要： | 采用荧光光谱法和紫外光谱法, 在生理条件下(pH=7.40)对氟比洛芬(FP)与脱氧核糖核酸(DNA)的作用方式进行了研究.证实了氟比洛芬通过沟槽结合方式与DNA发生相互作用.实验表明, DNA与氟比洛芬的相互作用为静态猝灭过程,测得结合常数为5.53×10~5 L·mol~(-1),结合位点数为1.12.
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| 关 键 词： | 氟比洛芬 荧光猝灭 相互作用 |
Study on the Interaction of Flurbiprofen with DNA by Fluorescence Quenching |
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| WANG Xiao-xia,AODENG Gao-wa,A Juan.Study on the Interaction of Flurbiprofen with DNA by Fluorescence Quenching[J].Journal of Analytical Science,2009,25(6). |
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| Authors: | WANG Xiao-xia AODENG Gao-wa A Juan |
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| Institution: | WANG Xiao-xia1,AODENG Gao-wa*1,A Juan2(1.College of Chemistry and Chemical Engineering,Neimongol University,Hohhot 010021,2.Department of Basic Curriculum,Agricultural University.Inner Mongolia,Hohhot 010019) |
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| Abstract: | The interaction of flurbiprofen and DNA in physiological condition was investigated by fluorescence spectrometry and UV absorption spectrophotometry. The results showed that flurbiprofen interacted with DNA in a groove binding mode, which resulted in the fluorescence quenching of flurbiprofen. The binding constant was 5.53×10~5 L·mol~(-1), and the binding site was 1.12. |
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| Keywords: | DNA Flurbiprofen DNA Fluorescence quenching Interaction |
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