| 高效液相色谱中苯甲醛的在线堆积研究及其应用 |
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| 引用本文： | 魏玉霞,王琳,肖盛元,邓玉林.高效液相色谱中苯甲醛的在线堆积研究及其应用[J].分析科学学报,2009,25(4). |
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| 作者姓名： | 魏玉霞 王琳 肖盛元 邓玉林 |
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| 作者单位： | 北京理工大学生命科学与技术学院,北京,100081 |
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| 基金项目： | 国家重点基础研究发展计划 |
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| 摘 要： | 以乙腈和水为流动相,研究了苯甲醛在苯基柱和C18柱双柱串联体系中的在线堆积行为.结果表明,苯甲醛在苯基柱-C18柱组合体系中能实现有效堆积,且速度差越大堆积得越好.在实验最佳堆积条件下,堆积因子为4.6,峰高增加5.4倍,该堆积条件下保留时间,峰面积,峰高和半峰宽的RSD值分别为1.2%, 4.2%,3.4%和0.80%.将苯甲醛的在线堆积效应用于单胺氧化酶(MAO)酶活性测定样品,信噪比提高了6.9倍,检测灵敏度显著提高.实际样品测定中保留时间,峰面积,峰高,半峰宽,塔板数和信噪比的RSD值分别为0.1%, 4.6%,5.0%,0.7%,1.2%和4.67%,精密度满足分析测定的要求.
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| 关 键 词： | 在线堆积 苯甲醛 灵敏度 高效液相色谱 |
On-Line Stacking Effect of Benzaldehyde in High Performance Liquid Chromatography and Its Application |
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| WEI Yu-xia,WANG Lin,XIAO Sheng-yuan,DENG Yu-lin.On-Line Stacking Effect of Benzaldehyde in High Performance Liquid Chromatography and Its Application[J].Journal of Analytical Science,2009,25(4). |
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| Authors: | WEI Yu-xia WANG Lin XIAO Sheng-yuan DENG Yu-lin |
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| Institution: | WEI Yu-xia,WANG Lin,XIAO Sheng-yuan,DENG Yu-lin(School of Life Science&Technology,Beijing Institute of Technology,Beijing 100081) |
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| Abstract: | The on-line stacking effect of benzaldehyde in high performance liquid chromatography(HPLC) based on the velocity difference of the solute on the different stationary phase was discussed by the coupling of two different stationary phases,using H2O and acetonitrile as mobile phase.The effective stacking was observed and the stacking factor increased with the increase of the velocity difference(the ratio of v1 to v2).Under the optimal experimental conditions,the stacking factor was 4.6,and the peak height inc... |
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| Keywords: | On-line stacking Benzaldehyde Sensitivity High perfomance liquid chromatography |
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