| 高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量 |
| |
| 引用本文： | 吴玉杰,陈冬梅,陶燕飞,袁宗辉,刘军义.高效液相色谱法测定动物组织中3-甲基喹喔啉-2-羧酸残留量[J].分析科学学报,2009,25(4). |
| |
| 作者姓名： | 吴玉杰 陈冬梅 陶燕飞 袁宗辉 刘军义 |
| |
| 作者单位： | 1. 广西出入境检验检疫局,广西南宁,530021
2. 华中农业大学国家兽药残留基准实验室,湖北武汉,430070 |
| |
| 基金项目： | 教育部"跨世纪优秀人才培养计划"，重点项目 |
| |
| 摘 要： | 本文建立了猪、鸡、鱼肌肉及猪、鸡肝脏组织中喹乙醇代谢残留标识物3-甲基喹喔啉-2-羧酸残留量的高效液相色谱分析方法.样品在酸性环境水解,经乙酸乙酯、磷酸盐缓冲溶液依次提取,Oasis MAX固相萃取柱净化,高效液相色谱紫外测定(HPLC-UV).3-甲基喹喔啉-2-羧酸标准曲线相关系数(r2)为0.9992(10～1 000 μg/L);不同动物组织样品中3-甲基喹喔啉-2-羧酸工作曲线相关系数(r)为0.9995～0.9997(2～100 μg/kg).在2～100 μg/kg浓度范围,3-甲基喹喔啉-2-羧酸回收率为69%～104%,日内相对标准偏差＜15%,日间相对标准偏差＜20%.检出限(CCα)为1.2～4.3 μg/kg,定量限(CCβ)为2.0～5.6 μg/kg.
|
| 关 键 词： | 喹乙醇 3-甲基喹喔啉-2-羧酸 固相萃取 高效液相色谱法 动物组织 |
Determination of the Marker Residue of Olaquindox,Methyl-3-quinoxaline-2-carboxylic Acid in Animal Edible Tissues Using High Performance Liquid Chromatography |
| |
| WU Yu-jie,CHEN Dong-mei,TAO Yan-fei,YUAN Zong-hui,LIU Jun-yi.Determination of the Marker Residue of Olaquindox,Methyl-3-quinoxaline-2-carboxylic Acid in Animal Edible Tissues Using High Performance Liquid Chromatography[J].Journal of Analytical Science,2009,25(4). |
| |
| Authors: | WU Yu-jie CHEN Dong-mei TAO Yan-fei YUAN Zong-hui LIU Jun-yi |
| |
| Institution: | WU Yu-jie1,CHEN Dong-mei2,TAO Yan-fei2,YUAN Zong-hui2,LIU Jun-yi1(1.Guangxi Entry-Exit Inspection and Quarantine Bureau,Nanning 530021,2.National ReferenceLaboratory of Veterinary Drug Residues Huazhong Agricultural University,Wuhan 430070) |
| |
| Abstract: | A method was developed for the quantitative determination of methyl-3-quinoxaline-2-carb-oxylic acid(MQCA),the metabolite that has been designated as the marker residue for olaquindox in swine tissue.This is the first report on simultaneous determination of MQCA in pork,chicken,fish and porcine,chicken livers.Samples were deproteinated with 5% metaphosphoric acid in 10% methanol followed by liquid-liquid extraction.Further clean-up was performed by solid phase extraction(SPE) through mixed mode anion-exchan... |
| |
| Keywords: | Olaquindox Methyl-3-quinoxaline-2-carboxylic acid Oasis MAX SPE High performance liquid chromatography Animal tissues |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
