| 适配体功能化多壁碳纳米管整体柱分离富集细胞色素c |
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| 引用本文： | 周婵媛,杜苑琪,杜卓,李攻科,肖小华.适配体功能化多壁碳纳米管整体柱分离富集细胞色素c[J].分析科学学报,2017,33(5). |
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| 作者姓名： | 周婵媛 杜苑琪 杜卓 李攻科 肖小华 |
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| 作者单位： | 中山大学化学学院,广东广州,510275 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金重点项目，广东省公益研究与能力建设专项，广州市科技计划 |
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| 摘 要： | 本文结合核酸适配体的特异性吸附及碳纳米管比表面积大的特点,研制了核酸适配体功能化碳纳米管/聚合物复合整体柱(AMMC),并将其应用于细胞色素c(Cyt c)选择性分离。结果表明,AMMC具有良好的通透性和稳定性,可选择性富集Cyt c,富集量可达1.47 mg。结合顺序注射系统,AMMC成功用于猪心样品中Cyt c的选择性分离富集,在生物样品分析中具有良好的应用前景。
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| 关 键 词： | 核酸适配体 碳纳米管 整体柱 细胞色素c 分离富集 |
Aptamer-modified Multi-walled Carbon Nanotubes Incorporated Monolithic Column for the Separation of Cytochrome c in Biological Samples |
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| ZHOU Chan-yuan,DU Yuan-qi,DU Zhuo,LI Gong-ke,XIAO Xiao-hua.Aptamer-modified Multi-walled Carbon Nanotubes Incorporated Monolithic Column for the Separation of Cytochrome c in Biological Samples[J].Journal of Analytical Science,2017,33(5). |
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| Authors: | ZHOU Chan-yuan DU Yuan-qi DU Zhuo LI Gong-ke XIAO Xiao-hua |
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| Abstract: | Efficient and sensitive separation methods are very important for biological study.In the present work,on the base of aptamer and multi-walled carbon nanotubes,an incorporated monolithic column(AMMC) was prepared and applied for the selective enrichment of cytochrome c.The AMMC exhibited good permeability,stability and selectivity,and a good adsorption capacity of 1.47 mg/mL for cytochrome c.Combined with sequential injection,cytochrome c in human plasma and pork heart was selectively obtained,showing good potential on the application of biological separation. |
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| Keywords: | Aptamer Carbon nanotubes Monolithic column Cytochrome c |
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