| 高效液相色谱-荧光检测BMVC对核酸酶的抑制作用 |
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| 引用本文： | 吴明和,汪泉.高效液相色谱-荧光检测BMVC对核酸酶的抑制作用[J].分析科学学报,2011,27(5):678-680. |
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| 作者姓名： | 吴明和 汪泉 |
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| 作者单位： | 1. 武汉大学生命科学学院仪器设备共享中心,湖北武汉,430072
2. 中国科学院动物研究所,北京,100101 |
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| 摘 要： | 本文利用荧光标记代替传统的同位素标记,用高效液相色谱法(HPLC)研究了端粒酶抑制剂BMVC浓度依赖的酶切抑制作用,得到了和聚丙烯酰胺(PAGE)凝胶电泳一致的结果.说明了该方法的可行性,也为高通量筛选提供了可能性.
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| 关 键 词： | G四链体 荧光标记 浓度依赖 高效液相色谱 |
Study on the Inhibition of Different Concentrations of BMVC on Exonuclease I by High Performance Liquid Chromatography |
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| WU Ming-he,WANG Quan.Study on the Inhibition of Different Concentrations of BMVC on Exonuclease I by High Performance Liquid Chromatography[J].Journal of Analytical Science,2011,27(5):678-680. |
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| Authors: | WU Ming-he WANG Quan |
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| Institution: | WU Ming-he*1,WANG Quan2(1.Sharing Center for the Equipment,College of Life Sciences,Wuhan University,Wuhan 430072,2.Institute of Zoology,Chinese Academy of Sciences,Beijing 100101) |
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| Abstract: | Human telomere DNA and a variety of guanine-rich sequences can fold into an intramolecular G-quadruplex structure.Traditionally,isotope labeling is used to analyse the inhibition effect of G-quadruplex on enzymes.In the present study,we employed fluorescence labeling instead of the idiomatic isotope labeling to study the small molecules concentration-dependent inhibition of exonuclease I by high performance liquid chromatography.The obtained results are in line with PAGE electrophoresis,which demonstrates t... |
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| Keywords: | G-quadruplex structure Fluorescence labeling Concentration-dependent High performance liquid chromatography |
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