乳品中金黄色葡萄球菌肠毒素B免疫传感检测方法的研究

本文利用自制的金黄色葡萄球菌肠毒素B(SEB)抗体构建了一种L-半胱氨酸和纳米金的双层自组装免疫传感器,采用循环伏安法及交流阻抗法对传感器进行表征与测定,并对各项相关条件进行优化,最终建立了SEB检测的标准曲线,线性范围分别在2～10 ng/mL和10～100 ng/mL,相关系数分别为0.9939和0.9926,检出限(S/N=3)为0.667 ng/mL,乳品检测回收率在84.3%～93.2%之间。该传感器特异性良好,稳定性好,可再生使用,可应用于乳品中SEB的快速检测。

Research of an Electrochemical Immunosensor for Detecting Staphylococcus Aureus Enterotoxin B in Dairy Products

Staphylococcus aureus enterotoxin B(SEB) is a kind of extracellular toxins which is secreted by Staphylococcus aureus and mainly exists in high protein foods,such as milk.In this paper,we constructed an self-assembly immunosensor with self-made SEB antibody,double-layer L-cysteine and gold nanoparticle.The sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.We also optimized the experimeutal conditions,and established the SEB standard curve.The linear ranges were 2~10 ng/mL and 10~100 ng/mL with a correlation coeficient of 0.9939 and 0.9926,respectively,and the detection limit was 0.667 ng/mL(S/N=3).The sensor shows good specificity,reusability and stability,and can be used in dairy products for the rapid detection of SEB.