| 茜素红可见分光光度法测氯霉素的含量 |
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| 引用本文： | 庞向东,舒亚林,徐杰,江虹.茜素红可见分光光度法测氯霉素的含量[J].分析科学学报,2012,28(2):253-256. |
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| 作者姓名： | 庞向东 舒亚林 徐杰 江虹 |
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| 作者单位： | 1. 长江师范学院化学化工学院,重庆,408100
2. 重庆市涪陵计量质量检测中心,重庆,408000 |
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| 基金项目： | 重庆市教委科技基金，长江师范学院科研项目 |
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| 摘 要： | 研究了氯霉素与茜素红之间的络合反应,确定了最佳反应条件,建立了一种快速、简便测定氯霉素含量的可见分光光度法。研究表明,在pH=11.41的B-R介质中,氯霉素与茜素红在室温条件下即可形成1∶1型络合物,该络合物的λmax=474 nm,表观摩尔吸光系数ε为1.19×104L/(mol.cm)。氯霉素的浓度在2.0×10-7～3.0×10-5mol/L范围内服从比耳定律,相关系数r为0.9985。测定结果的相对标准偏差为0.86%(n=10),回收率为99.4%～101.2%。
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| 关 键 词： | 氯霉素 茜素红 分光光度法 |
Spectrophotometric Method for the Determination of Chloramphenicol by Alizarin Red |
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| PANG Xiang-dong
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SHU Ya-lin
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XU Jie
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JIANG Hong.Spectrophotometric Method for the Determination of Chloramphenicol by Alizarin Red[J].Journal of Analytical Science,2012,28(2):253-256. |
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| Authors: | PANG Xiang-dong SHU Ya-lin XU Jie JIANG Hong |
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| Institution: | 1(1.College of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100; 2.Chongqin Fuling Center of Metrological Examination and Quality Inspection,Chongqing 408000) |
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| Abstract: | The complex reaction of chloramphenicol(CHP) and alizarin red(ALZR) was investigated by spectrophotometry.The optimum experimental conditions were established,and a simple and rapid method for the determination of chloramphenicol has been developed.Results showed that the ratio of chloramphenicol and alizarin red in the composition of complex was 1∶1 in Britton-Robinson(B-R)-ethanol solution at room temperature.The apparent molar absorptivity of the complex was 1.19×104 L/(mol·cm)at 474 nm.The Beer’s law was obeyed in the range of 2.0×10-7~3.0×10-5 mol/L for chloramphenicol with a correlation coefficient of 0.9985.The relative standard deviation was 0.86%(n=10),and the recovery was 99.4%~101.2%. |
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| Keywords: | Chloramphenicol Alizarin red Spectrophotometry |
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